中文摘要：

粗马铃薯多酚氧化酶固定于丝素／SiO2杂化膜上，从而得到丝素／SiO2杂化膜固定化多酚氧化酶，并用于处理模拟含酚废水。丝素／SiO2杂化膜在水中的溶失率小于丝素膜在水中的溶失率。所得丝素／SiO2杂化膜固定化多酚氧化酶的最适宜pH为7．4。相对于游离多酚氧化酶，该酶具有较好的热稳定性、贮存稳定性及重复使用性。研究表明，丝素／SiO2杂化膜固定化多酚氧化酶有实际处理含酚废水的可能。

英文摘要：

A hybrid membrane of silk fibroin/SiO2 （SF/SiO2） was prepared to renain the partially purified potato polyphenol oxidase （PPO） for removing a phenolic compound from the test solution. The hybrid membrane was less water soluble than the SF membrane. The optimum pH of immobilized PPO was about 7.4. This immobilized PPO exhibited a higher thermal stability, and it was more stable and more effective for continuous use than the free PPO. The SF/SiOz membrane with immobilized PPO was reusable after regeneration by water rinsing and thus may be cost effective for removing phenolic compounds from industrial wastewaters.