中文摘要：

[目的]了解toll样受体3（TLR3）与孕妊乙肝病毒（HBV）感染的关系。[方法]提取组织总RNA，逆转录成mRNA，采用qRTPCR（real-time quantitative，RT-PCR卖时荧光定量逆转录-多聚酶链反应相对定量的方法）分别检测正常孕妇和HBsAg阳性孕妇胎盘组织中TLR3 mRNA，从基因水平比较TLR3在两组表达的差异。[结果]实时荧光定量PCR技术结果显示，病例组TLR3 mRNA的含量低于对照组。病例组Ct比值（TLR3／β-aetin）为1.7029±0.20306,对照组为1.3882±0.19125,两组比较差异有统计学意义（t=-4.698，P=0.000 〈0.05）。[结论]胎盘组织中TLR3表达与孕妇HBV感染有一定关系。TLR3高表达可能是孕妇HBV感染的保护因素。

英文摘要：

[Objective]To explore the relationship of TLR3 in placenta and HBV infection of pregnant women. [Methods] Expressions of TLR3 mRNA in placentas of normal women and pregnant women with HBV infection were analyzed by real-time quantitative RT-PCR. Compared the different expressions in the two groups at the gene level. [ Results] The results from real-time fluorescence quantitative RT-PCR revealed that the content of TLR3 was lower in women with HBV infection than that in normal women. The difference had a statistical significance （t=-4.698, P = 0.000 〈 0.05）. [Conclusion] The expression of TLR3 has a association with HBV infection in pregnant women. High expression of TLR3 may effectively protect preg- nant women from HBV infection.