中文摘要：

目的研究人类白细胞抗原（HLA）-DR3、DR7、DR13、DR53等位基因与乙型肝炎病毒（Hepatitis Bvirus，HBV）宫内感染的关系，探讨FIBV宫内感染的遗传易感性，确定HBV富内感染的易感基因和保护基因。方法采用聚合酶链反应／序列特异性引物（PCR—SSP）技术检测HBsAg阳性孕妇及其新生儿各187例外周静脉血血块中HLA—DR3、DR7、DR13、DR53等位基因型分布及频率。结果（1）宫内感染组孕妇HLA—DR3基因频率为24．14％（7／29），显著高于非宫内感染组孕妇的6．33％（10／158）（P=0．007）。其余各表型比较，差异无统计学意义（P〉0．05）。（2）宫内感染组新生儿HLA—DR3基因频率为17．24％（5／29）。显著高于非宫内感染组新生儿的5．06％（8／158）（P=0．033）。其余各表型比较，差异无统计学意义（P〉0．05）。（3）母婴HLA—DR3同阳性在宫内感染组与宫内未感染组间比较差异有统计学意义（P=0．049）；母婴HLA-DR7、DR13、DR53同阳性在宫内感染组与宫内未感染组间比较。差异无统计学意义（P〉0．05）。结论HBsAg阳性孕妇及其胎儿同时或任一携带HLA—DR3，易导致HBV宫内感染；HLA—DR3是HBV宫内感染的易感基因。

英文摘要：

Objective To study the association between HLA- DR3, DR7, DR13, DR53 and HBV intrauterine infection to probe into the susceptibility to intrauterine infection of hepatitis B virus and to find its predisposing genes and protecting genes. Methods HLA - DR3, DR7, DR13, DR53 were detected by the technique of polymerase chain reaction - sequence specific primers（PCR - SSP） in maternal and neonatal blood clot. Results （1） The frequency of HLA - DR was 24.14 96 in women with intrauterine infection, which was higher than that （ 6.33 % ） in women without intrauterine infection （ P = 0. 007）. There was no association between HLA- DR7, DR13, DR53 positive in pregnant women and HBV intrauterine infection（ P 〉 0.05）. （2） The frequency of HLA- R of newborns was 17.24 % in women with intrauterine infection, which is higher that （5.06%） in women withotll infection（P = 0. 033）. There was no association between HLA - DR7, DR13, DR53 positive in newborns and HBV intrauterine infection（ P 〉 0.05 ）. （3） There was significant association between HLA - DR3 concordance and HBVintrauterine infection（P = 0. 049）. There was no association between DR7, DR13, DR53 concordance and HBV intrauterine infection（P 〉 0.05 ）. Conclusion HLA - DR3 carriers of pregnant women and their newborns have susceptibility to HBV intrauterine infection. HLA- DR3 is the predisposing gene of HBV intrauterine infection.