中文摘要：

目的研究乙型肝炎病毒（HBV）感染对人胎盘滋养层细胞系Bewo中TLR3 mRNA表达的影响，观察人胎盘滋养层细胞系Bewo中TLR3抗HBV感染的生物学作用。方法培养人胎盘滋养层细胞Bewo，利用PolyI：C刺激Bewo细胞TLR3mRNA的表达，同时设对照组，实时荧光聚合酶链反应（realtimeliT—PCR）检测细胞经PolyI：C刺激后TLR3mRNA的表达变化；同时以高浓度HBVDNA阳性血清与两组Bewo细胞共培养，荧光定量PCR法检测细胞上清液中HBVDNA含量的变化。结果Bewo细胞经PolyI：C刺激后TLR3 mRNA水平高于对照组（t=7．90，P〈0．01），差异有统计学意义；用HBV DNA阳性血清感染细胞时，培养上清中均可检出HBVDNA。PolyI：C组上清中HBVDNA含量低于对照组（t=7．088，P〈0．01），差异有统计学意义。结论PolyI：C刺激可使Bewo细胞TLR3 mRNA表达上调，与HBV共孵育后，培养上清中HBV病毒载量低于对照组，提示TLR3在人胎盘滋养层细胞系Bewo遭受HBV感染时发挥了一定的抗病毒作用。

英文摘要：

Objective To explore the role of Toll-like receptor 3 （TLR3） in Bewo, a human trophoblast cell line, against HBV infection in vitro. Methods Human trophoblast cell line （Bewo） was culture and stimulated to express TLR3 mRNA using PolyI：C. Quantitative real-time RT-PCR was employed to detect the expression of TLR3 mRNA level in Bewo cells with or with PolyI：C stimulation. Meanwhile, the experiment group and the control group were co-cultured with high-titer HBV DNA-positive serum. HBV DNA copies in culture superuatant were measured by fluorescence quantitative PCR （FQ-PCR）. Results Bewo cells showed higher levels of TLR3 expression after PolyI：C stimulation as compared with the control group （t=7.90,P〈0.01）. HBV DNA was detectable in supematant after co-culturing for 24 hours with HBV DNA positive sera. The concentrations of HBV DNA in Poty I：C group was significantly lower than the control group （t=7.088 ,P〈0.01）. Conclusion Poly I：C stimulation may up-regulate TLR3 mRNA in Bewo cells. The lower HBV load in supernatant of Poly I：C group suggested the anti-viral action of TLR3 in human trophoblast celll line Bewo against HBV infection.