利用Charleston×东农594构建的F2衍生的149个F2:14~F2:16株系组成的重组自交系群体,采用复合区间作图法(CIM)和多重区间作图法(MIM),连续3 a对一粒荚数、二粒荚数、三粒荚数和四粒荚数共4个荚粒性状进行QTL分析。结果表明:3 a间CIM和MIM分别定位到13个和24个QTL,分布11个连锁群上。采用CIM法,定位到1个控制一粒荚数的QTL在2 a中稳定出现;采用MIM法,定位到1个控制二粒荚数的QTL和1个控制四粒荚数的QTL在2 a中稳定出现,且控制四粒荚数的QTL 2 a的性状贡献率分别为72.5%和37.6%。
The population of 149 recombination inbred lines(RIL)derived from the cross of Charleston×Dongnong 594 were adopted.QTLs of one-seed pod,two-seed pod,three-seed pod and four-seed pod form 2006 to 2008 were analyzed by composite interval mapping(CIM)and multiple intervals mapping(MIM)method.Thirteen QTLs and 24 QTLs were mapped by CIM and MIM method,respectively.QTLs were located on 11 linkage groups,and 1 QTL of one-seed pod was mapped by CIM method in two years.One QTL of two-seed pod and 1 QTL of four-seed pod were mapped by MIM method in two years,and the QTL of four-seed pod in two years could explain 72.5% and 37.6% phenotypic variation,respectively.