中文摘要：

NF—Y（nuclearfactor-y）是真核生物中普遍存在的一种转录因子复合物，由3个不同的亚基（NF—YA、NF—YB和NF—YC）组成，并通过作用于其他调控因子的启动子来调节目的基因的表达。本文从大豆垦农18中克隆大豆NF—YC基因GmNF-YC2，并且利用实时荧光定量PCR（qRT-PCR）分析其表达模式，发现GmNF—YC2的表达受光调控，并在开花期第3复叶中表达量最高。结果表明，GmNF—YC2在大豆光周期开花调节中起重要作用。拟南芥原生质体瞬时表达实验证实，GmNF—YC2蛋白定位在细胞核中。这为大豆NF—YC家族基因的功能研究提供重要依据。

英文摘要：

The eukaryotic nuclear factor Y （NF-Y） is a ubiquitous transcription factor complex composed of three distinct subunits （NF-YA, NF-YB, and NF-YC）. NF-Y transcription factors typically act on the promoters of other regulatory factors to modulate gene expressions. Here, a NF-YC homologue, GmNF-YC2, was cloned from soybean variety KN18. The expression profiles checked by quantitative real-time RT-PCR （qRT-PCR） showed that GmNF-YC2 was regulated by light but not by circadian clock, and GmNF-YC2 was expressed at the highest level in third trifoliolates at flowering. The results suggested that GmNF-YC2 was one of the important genes in the regulation of flowering in soybean. The transient expression in Arabidopsis protoplasts displayed that GmNF-YC2 protein was localized in nuclear section. The results from this study pave a way to study the function of NF-Y family in soybean and its application in soybean molecular breeding.