中文摘要：

目的：界面法是一种通过插件将组织片静态地置于气液界面上进行培养的新型的技术手段，界面法组织培养的研究已成为近年热点，本文对界面法组织培养的定义、形成的历史、实施过程和特性、应用以及优缺点进行综述。 资料来源：应用计算机检索Medline数据库1991-01／2005-06期间的相关文章，检索词为“organotypic，culture，slice culture”，分别组合进行检索，并限定文章语言种类为English。同时计算机检索中国期刊全文数据库1991—01／2005-06期间的相关文章，检索词为“器官型培养，组织培养”，并限定文章语言种类为中文。 资料选择：对资料进行初审，并查看每篇文献后的引文。纳入标准：文章所述内容应与界面法组织培养研究相关。排除标准：重复研究或Meta分析类文章。 资料提炼：共收集到208篇相关文献，将其分类整理，筛出经典或近期发表的文章共26篇纳入分析。排除的182篇文献为内容陈旧或重复。符合纳入标准的26篇文献中，2篇涉及界面法组织培养的定义，3篇涉及界面法组织培养形成的历史，26篇涉及界面法组织培养的实施过程、特性与应用，7篇涉及界面法组织培养的优缺点。 资料综合：界面法是一种通过插件将组织片静态地置于气液界面上进行培养的新型的组织培养方法，从悬滴培养法、培养瓶培养法、灌注小室培养法，到目前仍广泛采用的旋转管培养法，造就了组织培养技术发展的里程碑。界面法的诞生弥补了上述方法某些方面的不足，为生命科学领域的研究提供了一个崭新的平台。界面法组织培养的实施过程所需设备简单，操作相对容易，并可以根据相应的实验目的来选择培养基（有无血清）和取材动物（年龄大小）。目前，在神经科学领域，已被广泛地应用于神经系统的发育、损伤与修复、神经药物的作?

英文摘要：

OBJECTIVE： The interface method is a new technique in which tissue slices are placed at the air-medium interface through inserts and keep stationary during the whole culture process. The investigation of the interface method has become the hotspot in recent years. In this article, we review its definition, history, operation, properties, applications, advantages and disadvantages. DATA SOURCES： The key terms of ＂Organotypic, culture, slice culture, tissue culture＂were respectively combined with each other while searching the Modline database for the relevant articles published from January 1991 to June 2005 in English. Meanwhile, we also searched China Journal Fulltext database for relative articles published from January 1991 to June 2005 ,with the key words of ＂organotypic,culture,slice culture＂ in Chinese. STUDY SELECTION： We examined all the materials firstly, then looked through the references of each one, Inclusive criteria： the articles related to the research of interface slice culture method. Exclusive criteria： repetitive researches or Meta analytical articles. DATA EXTRACTION： A total of 208 articles were collected and classifiod. Twenty-six ones, which were classical or had published recently, were selected. Altogether 182 articles were excluded due to repetitive or dated study. In these 26 articles according to the inclusive criteria, 2 were related to the definition of the interface culture method,3 related to its history, 26 related to its operation, properties and applications, and 7 related to its advantages and disadvantages. DATA SYNTHESIS： The interface method is a new technique that tissue slices are placed at the air-medium interface through inserts and keep stationary during the whole culture process. From hanging-drop, flask, perfusion chamber to widely-applied roller-tube culture method, they are mile stones in the development of tissue culture methods. And the naissance of the interface method, which provides a new stage for the investigations of lifescience, o