中文摘要：

目的：分析蛋白酶体抑制剂lactacystin（乳胞素）与多巴胺能神经元变性死亡的关系，为帕金森病的治疗探索新的思路。方法：实验于2005-03／11在国家人类基因组北方研究中心完成。实验细胞人神经母细胞瘤细胞系SH—SY5Y由北京神经科学研究所提供。用50μmol／L6-羟基多巴胺处理的多巴胺能神经细胞系SH—SY5Y作为帕金森病的细胞模型，于对数生长期时接种到培养皿、6孔板、96孔板中，24h后分不同组给药处理：对照组，50μmol／L6-羟基多巴胺组；50μmol／L6-羟基多巴胺加0．1μmol／L lactacystin组、50μmol／L6-羟基多巴胺加0．25μmol／L lactacystin组、50μmol／L6-羟基多巴胺加0．5μmol／L lactacystin组。镜下细胞计数，计算细胞存活率=活细胞数／对照组活细胞数×100％。磺酰罗丹明B法测定细胞括力，在酶联免疫检测仪测定吸光度值，检测波长为540nm。吸光度值=实验组细胞孔吸光度值-空白孔细胞吸光度值。取8复孔读数均值。细胞活性=实验孔吸光度值／平行对照孔吸光度值×100％结果：①50μmol／L6-羟基多巴胺组对细胞有明显毒性，细胞存活率与对照组相比只有（51,31±3．52）％，加用0．1，0．25，0．5μmol／L lactacystin后，细胞存活率分别提高到（72.16±97）％，（82.36±3．78）％，（91.44±3．06）％，各组之间差异有统计学意义（P〈0．05）。而单用0．5μmol／L的lactacystln对细胞存活率无明显影响。②50μmol／L6-羟基多巴胺组对细胞有明显毒性可表现为细胞数量的减少，6-羟基多巴胺组细胞存活率只是对照组的（47．33±3．25）％，加用0．1，0．25，0．5μmol／L的lactacystin后，细胞存活率分别提高到（69．67±2．13）％，（80．38±1．12）％，（90．59±2．01）％，各组之间差异有统计学意义（P〈0．05）。而单用0.5μmol／L的lactacystin对细胞存活率无明显影响。③正常培养的?

英文摘要：

AIM： To analyze the relationship between proteasome inhibitor （laetaeystin） and the degeneration and death of dopaminergie neurons, so as to develop a new therapeutic strategy for Parkinson disease. METHODS： The study was carried out in the Chinese National Human Genome Center （Beijing） between March and November 2005. The human neuroblastoma cell line SH-SY5Y was offered by Beijing Ipstitute of Neuroscienee. SH-SYSY cells treated with 50 μmol/L 6- hydroxydopamine （6-OHDA） were used as a cell model of Parkinson disease. The cells were plated to culture dishes, 6-well plate and 96- well plate in the logarithm growth period, and treated with different administrations after 24 hours： control group, 6-OHDA 50μmol/L group, 6-OHDA 50μmol/L ＋ laetacystin 0.1 μmol/L group, 6-OHDA 50μmol/L ＋ laetaeystin 0.25 μmol/L group, 6-OHDA 50μmol/L ＋ laetaeystin 0.5 μmol/L group. Cell survival rate =number of live cells/ number of live cells in the control group×100%. The absorbanee （A） value was detected with ELISA mieroplate reader, the wave length was 540 nm. A value= A of cell well in the experimental group-A of blank well. The mean of 8-well reading of each group was used to assay. Cell viability = A of experimental well/ A of parallel control well×100%. RESULTS： ①In SRB assay, 50 μmol/L 6-OHDA was toxic to cells, the cell survival rate was only （51.31±3,52）% as compared with that in the control group, and it increased to （72.16±3.97）%, （82.36±3.78）% and （91.44±3.06）% after 0.1, 0.25 and 0.5 μmol/L laetaeystin were added, and there were significant differences among the groups （P 〈 0.05）. But the treatment of 0.5 μmol/L laetaeystin only had no obviously influence on the cell survival rate. ②In cell count, 50 μmol/L 6-OHDA was toxic to cells, which was manifested by the reduce of cell number, the cell survival rate was only （47.33±3.25）% as compared with that in the control group, and it increased to （69.67±2.13）%, （80.38±1.12）%, （90.59±2