中文摘要：

目的研究α-突触核蛋白（α-Synuclein,α-Syn）在正常大鼠脊髓中的表达。方法利用2种抗α-Syn单抗3D5和2E3进行免疫组化染色,观察α-Syn在脊髓的表达和分布;用免疫荧光双标方法观察α-Syn与神经肽Y（NPY）共存的情况。用Luxol fast blue染色鉴别有髓与无髓神经纤维。结果α-Syn主要存在于脊髓灰质神经元核及灰质无髓神经纤维中。富含α-Syn的神经纤维主要分布在脊髓灰质的第Ⅰ、Ⅱ层,而α-Syn核阳性神经元主要分布在第Ⅶ、Ⅷ、Ⅸ、Ⅹ层;后根第Ⅰ、Ⅱ层一小部分α-Syn阳性纤维与NPY共存;前角细胞核呈α-Syn阳性神经元胞质中存在NPY,而其他部位的α-Syn核阳性神经元胞质中无NPY。结论α-Syn存在于大鼠脊髓灰质的神经元核及无髓神经纤维中,部分阳性神经纤维和神经元胞体呈NPY免疫阳性反应。

英文摘要：

Objective To provide a systematic map of α-synuclein（α-Syn） localization in the normal rat spinal cord. Methods Two home-made monoclonal antibodies （2E3 and 3 D5 ） against ot-Syn were immumohistochemically used to reveal the localization of α-Syn in the spinal cord. Luxol Fast Blue staining was applied to identify the unmyelinated nerve fibres. Double immunofluorescenee was used to observe the co-localization of α-Syn with neuropeptide Y （NPY）. Results α-Syn was localized in the neuronal nuclei and the unmyelinated nerve fibers within the gray matter. In both 2E3-and 3D5-stained sections, intensely stained nerve fibers were observed in laminae Ⅰ and Ⅱ. However, the immunopositive neuronal nuclei were only found in 3D5-stained sections, which were localized within the gray matter, especially in laminae Ⅶ,Ⅷ, Ⅸ, Ⅹ. Double immunofluorescent staining showed that some of the α-Syn-immunoreactive fibers and terminals in laminae Ⅰ and Ⅱ stained also for neuropeptide Y（NPY） in the dorsal horns, whereas in the anterior horns, a few motor neurons expressing α-Syn in the nuclei had cytoplasmic NPY. Conclusion α-Syn is localized in the neuronal nuclei and the unmayelinated nerve fibers within the gray matter of the spinal cord. Some of the α-Syn-positive neurons and fibers are NPY-positive.