欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的研究过氧化物酶体增殖物激活受体-γ2（PPAR-γ2）和过氧化物酶体增殖物激活受体-γ共激活蛋白1（PGC-1）α的单核苷酸多态性与多囊卵巢综合征（PCOS）发病及生殖激素的关系。方法收集PCOS患者和非PCOS妇女正常对照的血液标本。记录两组的初潮年龄,身高、体重,分离血清测定生殖激素,分离血液中的淋巴细胞提取总DNA,特异引物扩增后限制性酶切（PCR-RFLP）,电泳分离,溴化乙锭（EB）染色。结果PCOS患者的PPAR-γ2（Pro12Pro,Pro12Ala和Ala12Ala）基因型分布（分别为0.910,0.090,0）与正常人的分布（分别为0.925,0.068,0.007）无显著差异。PCOS患者的PGC-1α（Gly482Gly,Gly482Ser和Ser482Ser）基因型分布（分别为0.328,0.402,0.269）与正常人的分布（分别为0.333,0.374,0.293）无显著差异。体重指数（BMI）、血清总睾酮（T）和卵泡刺激素（FSH）、黄体生成素（LH）、LH/FSH、孕酮（P）和雌二醇（E2）在PPAR-γ2和PGC-1α各基因型之间无显著差异。与其他人种比较,中国人PPAR-γ2中Ala等位基因频率较低（4.4%）,但PGC-1α的频率较高（48%）。结论结果提示PPARγ2 Pro12Ala和PGC-1αGly482Ser这两种单核苷酸多态性与PCOS的发病无相关性。
英文摘要：
Objective： To investigate the polymorphisms of the peroxisome proliferator-activated receptor-γ （Pro12Ala） and its coactivator-1 （Gly482Ser） genes in Chinese women with polycystic ovary syndrome （PCOS）. Methods： Menarche age was recorded. Body mass indices （BMI） were calculated. Blood samples were obtained for single nucleotide polymorphism analyses and hormone measurements. Genotyping of the Pro12Ala polymorphism of peroxisome proliferator-activated receptor-T2 （PPAR-γ2） gene and the Gly482Ser polymorphism of the PPAR-γ2 coactivator striction fragment length polymorphism method. （PGC-1α） gene by the polymerase chain reaction-restriction fragment length polymorphism metho.Results. In general, Chinese population had lower PPAR-γ2 Ala allele frequency （4.4%）, but higher PGC-la Ser allele frequency （34.1%） as compared with other ethnicities. The genotypic distributions of PPAR-γ2 （Prol2Pro, Prol2Ala and Alal2Ala） in women with PCOS （0. 910, 0. 090, and 0, respectively） did not differ from those in controls （0. 925, 0. 068, and 0. 007, respectively）. Genotypic distributions of the PGC-1α （Gly482Gly, Gly482Ser and Ser482Ser） gene in women with PCOS （0. 328, 0. 402, and 0. 269, respectively） did not differ from the genotypic distributions in controls （0. 333, 0. 374, and 0. 293, respectively）. BMI, total testosterone and other reproductive hormones such as FSH, LH, LH/FSH, P and E2 were also similar between the different genotypes of both PPAR-γ2 and PGC-1α gene in the PCOS patients. Conclusion. Our data suggest there is no association of PPAR72 Prol2Ala and PGC-1α Gly482Ser variants with the occurrence of PCOS in Chinese population.
同期刊论文项目
同项目期刊论文
Different phenotypes of polycystic ovary syndrome by Rotterdam criteria are differently steroidogeni
Association between ACE gene I/D polymorphisms and hyper- androgenism in women with polycystic ovary
Association between CYP19 gene SNP rs2414096 Polymorphism and polycystic ovary syndrome in Chinese w
Association of the aspartic acid-repeat polymorphism in the asporin gene with age at onset of knee o
Cystic fibrosis transmembrane conductance regulator (CFTR) gene 5T allele may protect against prosta
VEGF C-634G polymorphism is associated with protection from isolated ventricular septal defect: case
Lack of association between Interleukin-1a gene (IL-1a) C (-889) T Variant and Polycystic Ovary Synd
Lack of an association between CYP1A1 gene Ile462Val polymorphism and polycystic ovary syndrome in C
Effect of metformin pretreatment on pregnancy outcome of in vitro matured oocytes retrieved from wom
期刊信息
位置：