中文摘要：

探讨在卵巢自身胰岛素抵抗情况下,颗粒细胞增殖能力、雌激素合成能力的改变。方法是以猪卵巢颗粒细胞作为体外研究对象,利用磷酯酰肌醇-3激酶（PI-3K）特异性抑制剂——渥曼青霉素（Wortamanni,WT）人工诱导胰岛素抵抗的细胞模型。结果显示：①处理组细胞的葡萄糖摄取能力明显低于正常组细胞的葡萄糖摄取能力（756.25±158.81cpm/2×10^4cellsvs1144.75±81.09cpm/2×10^4cells,p〈0.05）;②处理组细胞中有丝分裂激活蛋白激酶1（MAPK1）和MAPK2蛋白的表达水平高于对照组,而该蛋白磷酸化p-MAPK蛋白的表达低于对照组;③处理组卵巢细胞MAPK、增殖细胞核抗原（PCNA）、血管内皮生长因子（VEGF）mRNA的表达（相对灰度）升高;④在胰岛素抵抗的状态下,雌激素以及芳香化酶的表达显著上调。结论为卵巢颗粒细胞的自身胰岛素抵抗,明显提高其分裂增殖能力和雌激素合成效能,该机制可能是PCOS卵巢在超排过程中易发卵巢过度刺激综合的重要机制。

英文摘要：

This paper investigates the effect of insulin resistance on proliferation of ovarian granulose cell and synthesis of steroid hormone, Method： Ovarian granulose cells from porcine follicles were isolated and cultured in vitro, and insulin resistance was induced by treatment with wortmannin. Insulin resistance was confirmed by impaired [^3H] labeled glucose uptake, The expressions of mitogenactivated protein kinase （MAPK）, proliferation cell nuclear antibody （PCNA）, vascular endothelial growth factor （VEGF）, cytochrome 450 aromatase （Aroma） by RT-PCR as well as MAPK1, MAPK2, p-MAPK by western blot were analyzed. Steroidogeneis was evaluated by RIA in medium of cell culture. Results： （1） Wortmannin treated cells had significant lower [^3H] labeled glucose uptake under the insulin stimulation （756.25 ±158.81 cpm /2 ×10^4cells vs 1144.75 ±81.09 cpm /2×10^4cells cells p 〈0.05） as compared with the Con group. （2） The expressions of MAPK1, MAPK2 protein were higher than that in the Con group. In contrast, the level of p-MAPK protein was lower than that in Con group. （3） The RT-PCR results show that the mRNA expressions of MAPK, PCNA, VEGF under the circumstance of IR were increased. （4） The expression of aromatase was increased as well as estradiol （E2） level in IR cells medium. Conclusions： Selective IR induced by Wortmannin in granulosa cells is related with the enhanced mitogenic potentials, which is the key biological factor of OHSS. Thus IR as a metabolic phenotype in polycystic ovary itself may contribute to its proliferative likehood of OHSS, a serious complication during ovulation induction.