中文摘要：

目的：检测蛋白激酶B2（Akt2）基因缺失对睾丸生殖功能的影响，探讨隐丹参酮对Akt2基因缺失雄性小鼠生殖功能的调控及其分子机制。方法：①雄性Akt2^-/-纯合子小鼠29只，Akt2^＋/＋野生型小鼠15只。将Akt2^＋/＋小鼠随机分为2组：A（Akt2^＋/＋，基础组，n=7）、B（Akt2^＋/＋，刺激组，n=8）；Akt2^-/-小鼠随机分为4组：C（Akt2^-/-，基础组，n=7）、13（Akt2^-/-，刺激组，n=8）、E（Akt2^-/-，溶剂组，n=7）、F（Akt2^-/-，隐丹参酮组，n=7）；②B组、D组行人绒毛膜促性腺激素（hCG）兴奋试验，5IU／20g，A组、C组予等体积的生理盐水，刺激4h后取材；F组给予中药隐丹参酮治疗，600mg／kg每日2次灌胃，连续8周，E组予等体积的溶剂，A、B、C、D组测随机血糖，E及F组治疗前后分别行口服葡萄糖耐量实验（OGTT）2g／kg体重。③称取各组体重和双侧睾丸重量，测血清睾酮（T）水平，RT—PCR法检测各组睾丸甾体激素合成酶及糖代谢相关基因表达水平。结果：①随机血糖测定发现C、D组小鼠血糖显著高于A、B组小鼠[（10．38±1．42），（10．96±1．81）mmol／Lvs（7．92±0．63），（8．32±0．44）mmol／L，P〈0．05]，OGTY实验发现，治疗前后E组和F组0、30、60及120min血糖值均无显著差别（P〉0．05）；②体重各组之间无显著差别（P〉0．05），C、D组睾丸重量显著高于A、B组[（0．17±0．01），（0．17±0．01）gVS（0．15±0．02），（0．15±0．01）g，P〈0．05]，E、F组睾丸重量无显著差别。血清T水平C组显著高于A组[（9．08±1．59）nmol／LVS（6．42±0．95）nmol／L，P〈0．05]，F组显著低于E组[（5．94±0．49）nmol／Lvs（8．18±1．44）nmol／L，P〈0．05]；③RT—PCR检测，基础状态下C组小鼠睾丸组织中Cyp11、Cyp17、3D—HSD、Star、Gsk3β、Erk-1、MCM2mRNA的表达水平显著高于A组小鼠（P〈0．05）；hCG刺激后B组和D?

英文摘要：

Objective： To investigate the impact of protein kinase B （Akt2） allele deletion on testicular reproductive function,and to discuss the regulatory effect of Cryptotanshinone on the reproductivity of male mice with Akt2 allele deletion and its molecular mechanism. Methods： Fifteen Akt2 ＋/＋ male mice were randomly divided into Groups A （baseline control, n = 7） and B （stimulation, n = 8）, and another 29 Akt2-/- male mice into C （basehne control, n = 7）, D （stimulation, n = 8）, E （solvent, n = 7） and F （ Cryptotanshinone, n = 7）. Groups B and D underwent human chorionic gonadotropin （HCG） stimulation tests at 5 IU / 20 g, while A and C received physiological saline, all for 4 hours; Group F were given gastric lavage of Cryptotanshinone, while E solvent only, at 600 mg/kg twice a day for 8 weeks, both subjected to oral glucose tolerance tests （OGTY） at 2 g/kg before and after the treatment. The body and bilateral testis weights were obtained, the serum testosterone （T） level measured, and the expressions of testicular steroid hormone synthesis and glycometabolism-related genes determined by RT-PCR. Results ： OGTF showed that the level of blood glucose was significantly higher in Groups C and D than in A and B （ [ 10.38 ± 1. 42] and [ 10.96 ± 1. 81 ] mmol/L vs [7.92 ±0.63 ] and [ 8.32 ± 0.44] mmol/L, P 〈 0. 05）, but had no significant differences at different time points in E and F （P 〉 0.05）. The testis weight was remarkably higher in Groups C and D than in A and B （ [0.17 ± 0.01 ] and [0.17 ± 0. 01 ] g vs [0.15 ± 0. 01 ] and [0.15± 0.02] g, P 〈0.05）, but exhibited no obvious difference in E and F, nor were there any significant differences in body weight among different groups （P 〉 0. 05）. The serum T level was markedly higher in Group C than in A （ [ 9.08 ±1.59 ] nmol/L vs [6.42 ± 0.95] nmo/L, P〈0.05）, but evidently lower in F than in E （[5.94 ± 0.49] nmol/L vs [8.18±＋ 1.44] nmol/L, P 〈 0.05）. The baseline expression