建立了体外的卵巢胰岛素抵抗模型。从卵巢内分子水平探讨胰岛素信号传导和促性腺激素信号传导的相互关系,揭示多囊卵巢综合征患者糖代谢异常和生殖功能障碍的关系;通过药物筛选得出两味中药增敏剂,与西药增敏剂进行了对照研究,探讨了其对卵巢颗粒细胞功能的影响。
Ovarian granulosa cells from porcine follicles were isolated and cultured in vitro for determination of the insulin resistance, by expressions of insulin signal molecules and gonadotropin-releasing hormone signal transduction, and to establish the relations between glucose metabolic disorders and reproductive dysfunction in polycystic ovary syndrome. A comparison was made between insulin-sensitizing western medicines and two traditional Chinese medicines berberine (Ber) and daidzein (Dai) in their improvement effects on insulin sensitivity, and the effects on function of the ovarian granulose cells.