中文摘要：

目的：研究金叶女贞果实花青素抗炎镇痛的作用，为进一步开发新药提供理论依据。方法：实验动物共240只，分为6项实验，每项实验40只，分为5组（n=8）：生理盐水对照组，阿司匹林对照组，花青素高、中、低剂量组。通过小鼠热板法和醋酸扭体法观察花青素镇痛作用；通过二甲苯至耳廓肿胀法、腹腔毛细血管通透性及棉球肉芽肿实验观察花青素的抗炎作用。测定小鼠血清中超氧化物歧化酶（SOD）、一氧化氮（NO）、总抗氧化能力（T-AOC）和前列腺素E2（PGE2）水平和小鼠肝脏匀浆的SOD、T-AOC及谷胱甘肽过氧化物酶（GSH-Px）酶活力，观察肝脏组织学变化。结果：中、高浓度的花青素能提高小鼠痛阈，减少扭体次数；花青素处理组小鼠血清SOD活性增高，NO和PGE2含量降低。高浓度花青素可抑制小鼠耳廓肿胀、腹腔毛细血管通透性的增加和肉芽肿增生，同时提高血清SOD和T-AOC活性，降低血清PGE2含量。肝脏SOD、T-AOC、GSH．Px酶活力有所提高，肝脏组织学切片在各组中未见明显变化。结论：金叶女贞果实花青素具有抗炎镇痛作用，其机制可能与提高小鼠抗氧化能力，减少NO和炎性因子PcB的生成有关，在实验浓度范围内连续给药7d对肝脏无明显损伤作用。

英文摘要：

Objective： To provide a theoretical basis for the further development of new drugs, the analgesic and anti-inflammatory effects and the liver function in mice of anthocyanin from Ligustnan vicaryi were investigated. Methods： The 240 experimental mice were splitted e- qually for 6 kinds of experiments and dO rats in each kind of experiment were divided into 5 groups （ n = 8） ： normal saline control group （NS） ; aspirin control group （Asp） ; high-concentration anthocyanin group （HA） ; medium-concentration anthocyanin group （MA） ; low-concentration anthocyanin group （LA）. The analgesia effect of anthocyanin at different concentration was determined by hot-plate test and acetic acid writhing test, and the anti-inflammatory effect of anthocyanin was performed by ear edema, abdomen capillary permeability and cotton granuloma. The activities of superoxide dismutase （SOD） and total antioxidant capacity（T-AOC） and the contents of nitric oxide （NO） and prostaglandin F2 （PGF2） in blood serum were determined, and the activities of SOD, T-AOC and glutathione peroxidase （GSH-Px） in liver were measured, while the histological changes of liver tissue were observed. Results： The pain threshold of mice was enhanced and the times of twist body was decreased by medium-concentration and high-concentration anthocyanin. The activity of SOD was increased and the contents of NO and PGF2 were reduced in blood serum. High-concentration anthocyanin inhibited the ear swelling , the increase of celiac capillary permeability and the granuloma hyperplasia, and increased the activities of SOD and T-AOC while decreased the content of PGE2. The activities of SOD, T-AOC, GSH-PX were increased in liver, but the morphology of liver tissues in each group had no significant changes. Conclusion： Anthocyanin of Ligustrmn vicaryi has definite anti-inflammatory and analgesic effects, which is related to increasing the antioxidant capacity and decreasing the contents of NO and PGF2, and has not obvious d