中文摘要：

目的：探讨硫化氢对糖尿病大鼠皮肤溃疡创面愈合的影响。方法：将30只健康雄性SD大鼠,随机分为正常对照组（NDC组）、糖尿病对照组（UDC组）和硫化氢处理组（TDA组）,每组10只。腹腔注射链脲佐菌素（65 mg/kg）柠檬酸缓冲液（p H 4.5）建立糖尿病大鼠模型。1周后,将正常大鼠及糖尿病大鼠背部剪开直径为20mm的圆形切口,正常组和糖尿病组大鼠的溃疡创面以空白基质处理,硫氢化处理组则用2%的硫氢化钠涂抹溃疡创面。分别在给药后第5、10、15、20天测定伤口愈合率。21 d后处死大鼠,分别收集血浆以及肉芽组织,检测血浆的凝血酶原时间（prothrombin time,PT）、凝血酶时间（thrombin time,TT）及纤维蛋白原（fibrinogen,FIB）,白细胞和淋巴细胞计数,ELISA检测血清超氧化物歧化酶（SOD）活性和丙二醛（MDA）的含量,Western blot检测肉芽组织中血红素加氧酶1（HO-1）和肿瘤坏死因子α（TNF-α）的表达水平;肉芽组织进行HE染色,光镜观察组织形态的变化。结果：与糖尿病组比较,硫氢化钠处理明显加快大鼠伤口愈合（P〈0.01）,提高了血清SOD活性（P〈0.01）,同时降低了MDA水平（P〈0.01）;血液学检测显示,硫氢化钠明显降低白细胞及淋巴细胞数量,延长PT及TT,降低FIB水平。病理切片观察显示,糖尿病对照组大鼠肉芽组织有炎症细胞浸润,胶原纤维排列疏松且不规则,而硫化氢组大鼠肉芽组织中胶原排列整齐,血管生成增多（P〈0.05）。此外,TDA组HO-1表达明显高于UDC组,而TNF-α的水平则显著低于UDC组。结论：硫化氢可促进糖尿病大鼠伤口愈合,其机制可能与其提高抗炎及抗氧化作用、改善微循环进而修复组织损伤有关。

英文摘要：

AIM： To study the effect of hydrogen sulfide on wound healing of skin ulcer in diabetic rats . METHODS：Male SD rats were randomly divided into 3 groups, including non-diabetic control （NDC） group, untreated diabetic control （ UDC） group, and treated diabetic administration （ TDA） group.Diabetic rats were induced by intraper-itoneal injection of streptozotocin （STZ）.After 1 week, wound healing model was prepared by making a round incision （ 2.0 cm in diameter ） on the dorsal skin in full thickness .The rats from TDA group received 2%sodium bisulfide ointment on the skin ulcer wound , and the animals from UDC and NDC groups received control cream .After 21 d of treatment with sodium bisulfide , blood samples were collected for biochemical analysis , including prothrombin time （ PT） , thrombin time （TT）, and fibrinogen （FIB） in plasma, as well as the activity of superoxide dismutase （SOD） and the content of malondi-aldehyde （ MDA） in the serum.White blood cells （ WBC） and lymphocytes were also counted .Granulation tissues from the wound were processed for histological examination and Western blot analysis was used to detect heme oxygenase -1 （ HO-1） and tumor necrosis factor α（TNF-α） expression.RESULTS：Compared with UDC group, sodium bisulfide treatment accelerated wound healing of skin ulcer （P〈0.01）, and increased the activity of SOD in serum （P〈0.01） in the diabet-ic rats.The declined number of WBC and lymphocytes , prolonged PT and TT , and decreased FIB levels in rats treated with sodium bisulfied were also confirmed .Pathological section showed that there were inflammatory cell infiltration , and irregu-lar and loose fibril alignment in the granulation tissue of rats from the UDC group , but there were regular fibril alignment and increased angiogenesis in the granulation tissue of rats from the TDA group （P〈0.05）.Furthermore, sodium bisulfide treatment raised HO-1 protein expression , and decreased TNF-αprotein expression in the di