目的:研究H2S对油酸导致的大鼠肺水肿的作用及其机制。方法:32只SD大鼠平均分成4组:对照组(CG组)、模型组(MG组)、预防组(PG组)、NaHS组(SG组)。CG组尾静脉注射生理盐水0.2mL/100g后,再注射生理盐水7μL/100g。MG组尾静脉注射生理盐水0.2mL/100g后,再注射油酸7μL/100g。PG组尾静脉注射NaHS 3.4mg/kg后,再注射油酸7μL/100g。SG组尾静脉注射NaHS3.4mg/kg后,再注射生理盐水7μL/100g。各组大鼠注射药物40min后颈总动脉取血并处死,取肺脏。观察肺脏大体观及肺组织病理改变,计算肺组织湿干重比,测定血清及肺组织丙二醛(MDA)水平、超氧化物歧化酶(SOD)活力。结果:与CG组相比,MG组大鼠肺脏包膜下淤血严重,肺脏表面大量片状出血、灶性实变及梗死;肺泡间隔增宽,肺泡壁损伤、断裂,肺泡内存在大量粉红色液体及圆形气泡;肺湿干重比(P〈0.05)及血清、肺组织中MDA水平均明显增加(P〈0.01),血清与肺组织SOD活力显著降低(P〈0.01)。与MG组相比,PG组大鼠肺脏仅见肺底部仍存在片状出血;肺组织病理结构显示间质轻度增厚,部分肺泡内存在粉红色液体,肺泡内圆形气泡少见,肺脏水肿程度较轻,湿干重比减小(P〈0.05);血清与肺组织中MDA水平降低(P〈0.01),而血清与肺组织中SOD活力则明显增高(P〈0.01)。与CG组比较,SG组各项指标差异均无统计学意义。结论:H2S能显著减轻肺组织损伤,减少血浆渗出量,具有减轻肺水肿的作用,其机制可能与增强机体的抗氧化能力,减轻肺组织的氧化损伤以及降低血管压力有关。
AIM:To study the effect and mechanism of H2 S in rats with pulmonary edema induced by oleic acid.METHODS:Thirty two rats were randomly divided into four groups:control group(CG),the rats ware injected with normal saline 0.2 mL/100 g intravenously followed by intravenous of normal saline 7uL/100 g.Model group(MG),the rats were injected normal saline 0.2mL/100 g intravenously,then injected with oleic acid 7uL/100 g.Prevention group(PG),the rats were injected with NaHS3.4mg/kg,then injected by equal amount of oleic acid intravenously and NaHS group(SG),the rats were injected with NaHS 3.4 mg/kg,then injected normal saline 7uL/100 g intravenously.After 40 min,the rats were killed.blood and lung were got for analyzing the level of MDA and the activity of SOD,observing lung pathologic gross and pathologic changes,calculating wet and dry weight ratioRESULTS:Compared with the CG group,flaky bleeding was emerged in the lung surface,focal consolidation and infarction and subcapsular congestion seriously in MG group.Oleic acid increased the thickness of alveolar septal,damaged the alveolar wall,and made alveoli full of pink secretion and round bubbles.Moreover,W/D and the level of MDA in blood plasma and lung tissue in MG group were increased obviously compared with those in CG group.But the activity of SOD decreased significantly.Lung surface only emerged flaky bleeding on the lower lung fields,slight interstitial thickening,minority alveolar exited pink secretion in PG group.W/D and the level of MDA in plasma and lung tissue in PG group were decreased obviously compared with those in MG group.The activity of SOD was increased significantly in PG group.Meanwhile,indicators of SG group have no significant differences.CONCLUSION:H2S has the effects of alleviating the lung injury,decreasing plasma exudation,and mitigating pulmonary edema.The mechanism may be correlation with enhancing the antioxidation,alleviating oxidative damage of lung tissue and lowering blood pressure.