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中文摘要：
目的 探讨粉尘螨Ⅲ类重组变应原（rDer f 3）对过敏性哮喘小鼠的免疫治疗效果。方法 随机将40只BALB/c小鼠均分为4组,哮喘组、免疫治疗组、卵清蛋白（OVA）组和PBS组,分别在第0、第7和第14天哮喘组和免疫治疗组每鼠经腹腔注射100μl致敏液（含rDer f 3 10μg）;卵清蛋白组每鼠经腹腔注射100μl致敏液（含OVA 10μg）;PBS组则以PBS代替致敏液。第21天起,哮喘组和免疫治疗组小鼠用rDer f 3进行滴鼻激发试验,连续7 d,免疫治疗组小鼠在第25～27天滴鼻激发前30 min,用100μg rDer f 3纯化蛋白皮下注射进行特异性免疫治疗。PBS组和卵清蛋白组则分别用PBS和OVA进行滴鼻激发和腹腔注射,最后1次滴鼻激发24 h后脱臼处死小鼠。收集各组小鼠支气管肺泡灌洗液（BALF）进行白细胞和嗜酸粒细胞计数;对肺组织病理切片进行HE染色,镜下观察肺组织炎症细胞浸润情况。ELISA检测BALF和脾细胞培养上清（SSCS）中白细胞介素-5（IL-5）和γ干扰素（IFN-γ）及血清中变应原特异性IgE、IgG2a抗体水平。结果 肺组织病理切片结果显示,免疫治疗组小鼠炎症反应明显减轻。小鼠BALF中白细胞总数在免疫治疗组、卵清蛋白组和哮喘组分别为（7.03±1.38）×108/ml、（22.11±3.70）×108/ml和（22.75±3.24）×108/ml,免疫治疗组低于卵清蛋白组和哮喘组（P〈0.01）,嗜酸粒细胞的变化趋势与白细胞类似。免疫治疗组、卵清蛋白组和哮喘组BALF中IL-5的水平分别为（108.20±11.02）pg/ml、（182.04±13.94）pg/ml和（195.33±15.33）pg/ml,SSCS中IL-5的水平分别为（98.34±13.06）pg/ml、（208.26±10.63）pg/ml和（179.54±13.65）pg/ml,免疫治疗组均明显低于卵清蛋白组和哮喘组（P〈0.01）。而IFN-γ含量则高于卵清蛋白组和哮喘组（P〈0.01）。IgE水平免疫治疗组、卵清蛋白组和哮喘组分别为（9.12±3.78）IU/ml、（26.87±4.30）IU/ml和（35.25±8.84）IU/ml,与卵清蛋?
英文摘要：
Objective To explore the effect of specific immunotherapy with major 3 group recombinant allergen rDer f 3 of Dermatophagoides farinae in murine asthma model. Methods Forty BALB/c mice were randomly divided into4 groups, namely PBS group（negative control）, ovalbumin（OVA） group（positive control）, rDer f 3 allergen sensitization group（asthma group）, and rDer f 3 specific immunotherapy group（SIT group）. The mice in asthma group and SIT group were injected intraperitoneally with purified rDer f 3 protein on days 0, 7 and 14, respectively, and rDer f 3 solution was inhalated from day 21 for 7 days. During the 25th-27 th day, mice in SIT group were injected subcutaneously with100 μg rDer f 3 allergen for 30 min before nasal inhalation. Mice in groups of PBS and OVA were treated with PBS and OVA, respectively. Twenty-four hours after the final challenge, all mice were sacrificed, the bronchoalveolar lavage fluid（BALF） was collected and the total number of white blood cells and the number of eosinophils were recorded. The levels of IL-5 and IFN-γ in BALF and supernatant of cultured splenocytes were detected by ELISA, as well as the serum levels of specific IgE and IgG2 aantibodies. Lung tissues were stained with haematoxylin and eosin for histological analysis. Results Compared with the asthma group, the rDer f 3-induced lung inflammation was significantly alleviated in SIT group. The total number of white blood cells [（7.03±1.38）×108/ml] in SIT group was considerably lower than that of OVA group [（22.11±3.70）×108/ml] and asthma group [（22.75±3.24）×108/ml]（P〈0.01）. The change trend of eosinophil leukocytes was similar with that of white blood cells. IL-5 levels in BALF [（108.20±11.02） pg/ml] and splenocyte culture supernatant [（98.34±13.06） pg/ml] in SIT group were significantly lower than that of OVA group [（182.04±13.94）pg/ml,（208.26±10.63） pg/ml] and asthma group [（195.33±15.33） pg/ml,（179.54±13.65） pg/ml]（P〈0.01）. Whereas, the level
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