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野生坛紫菜种群遗传多样性的ISSR分析
  • ISSN号:1000-0615
  • 期刊名称:水产学报
  • 时间:0
  • 页码:556-563
  • 语言:中文
  • 分类:S917[农业科学—水产科学]
  • 作者机构:[1]集美大学水产学院,福建厦门361021
  • 相关基金:国家自然科基金(40676077);国家海洋863计划(2006AA10A413);福建省科技重大专项(2004NZ03);福建省自然科学基金(2007J0064)
  • 相关项目:坛紫菜种质资源遗传分析、纯系培育及种质库构建
中文摘要:

采用简单序列重复区间(ISSR)分子标记技术对福建省的3个野生坛紫菜(Porphyra haitanensis)种群共60个个体进行了遗传多样性分析。15条引物共扩增出222个位点,其中186个位点具有多态性,多态位点百分率为83.789/6,在种群水平上,多态位点百分率为80.18%~81.53%,平均为80.93%。期望杂合度、Shannon信息指数在物种水平上分别为0.3304和0.4834;在种群水平上分别为0.3089和0.4551,表明坛紫菜种群内存在着较高的遗传多样性水平,且在三个种群间没有明显差异。依据Gst值,坛紫菜的遗传变异主要发生在种群内的个体间(占93.5%),只有6.5%的遗传变异发生在种群间,由此说明坛紫菜种群间的遗传分化水平很低,这与坛紫菜种群间充分的基因交流(Nm=7.1930)是相关的。基于遗传距离的UPGMA聚类结果表明坛紫菜60个个体并不按照其地理分布进行分群,而是随机分群的。文章最后还对坛紫菜种质资源保护的必要性进行了讨论。

英文摘要:

Porphyra haitanensis is a unique seaweed species that grows in South China and has the highest production (by weight) in Chinese aquaculture. However it has been a great loss in nori farming due to the degeneration of the germplasm quality in the last years. In order to understand the genetic background of P. haitanensis and guide its genetic breeding and germplasm resource protection, Inter-simple sequence repeats (ISSR) markers were used to investigate the genetic variations within and among 3 populations (PT, NR and DS) of P. haitanensis. The thalli samples were collected from 60 individuals. 15 ISSR primers gave rise to 222 discernible DNA bands of which 186 (83.78%) were polymorphic, indicated a considerable genetic variation at specific level. At the population level, the percentage of polymorphic bands (P) was from 80. 18% to 81.53%, 80. 93% on average. Expected heterozygosity (h) and Shannon's information index (I) were 0. 3304 and 0. 4834 respectively at specific level, 0. 3809 and 0. 4551 at population level. According to the value of Get, a large proportion of genetic variance (93.5%) of P. haitanensis was among individuals within population, only 6. 5% genetic variance was among populations, the results indicated that the genetic differentiation level among the 3 populations of P. haitanensis was low. And according to the value of Nm, the reason of the low genetic differentiation level among populations of P. haitanensis was the sufficient gene flow. UPGMA cluster analysis based on Nei's genetic distance showed that the individuals from the same population were no grouped together, but randomly. At Last, the necessary of protecting the germplasm resource of P. haitanensis also was discussed.

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期刊信息
  • 《水产学报》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国水产学会
  • 主编:黄硕琳
  • 地址:上海市军工路334号上海水产大学
  • 邮编:201306
  • 邮箱:jfc@shou.edu.cn
  • 电话:021-61900228
  • 国际标准刊号:ISSN:1000-0615
  • 国内统一刊号:ISSN:31-1283/S
  • 邮发代号:4-297
  • 获奖情况:
  • 曾先后三次获中国科协专项基金资助,获首届《CAJ-CD规范》执行优秀奖,获水产类期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:26128