采用20对简单重复序列(SSR)引物对福建省坛紫菜种质资源库中保存的44个坛紫菜种质材料进行了遗传分析,共检测到了81个多态性位点,每对引物检测到的等位基因介于2~6个之间,平均为4.05个,引物的PIC值介于0.15~0.79之间,平均为0.57。然后根据3对引物(Phes08,Phes03和PC13)扩增出的16个多态性位点构建了44个坛紫菜种质材料的指纹图谱库,使得每一种质材料均具有其特异的DNA指纹图谱。并根据指纹图谱中各扩增位点条带的有无,将指纹图谱转化成了计算机可以识别的数码指纹,开发了相应的数码指纹识别软件,为坛紫菜种质的自动化鉴定及坛紫菜种质资源库的信息化管理奠定了基础。
20 simple sequence repeat (SSR)primer-pairs were used to analyze the genetic diversity of 44 germplasm materials of Porphyra haitanensis stored in the germplasm repository. A total of 81 alleles were detected,with the number of alleles ranging from 2 to 6, and the average was 4.05, and the polymorphism information content(PIC) was between 0.15 and 0.79, with the average of 0.57. Then the 16 polymorphic alleles amplified from 3 primer-pairs of SSR (Phes08, Phes03 and PC13 )were used to construct the DNA fingerprinting dabatase of the 44 germplasm materials of P. haitanensis. In the constructed DNA fingerprinting database, each germplasm material has its unique fingerprinting pattern and can be easily distinguished from each other. At last the DNA fingerprints were transformed into digital fingerprints which can be recognized by computer based on appear ance and disappear ance of amplified band in each allele, and developed the corresponding software of digital fingerprints recognizer to predigest the germplasm identified process. In conclusion, the fingerprinting database will provide a basis for germplasm autoidentification and digital management of germplasm repository of P. haitanensis.