中文摘要：

客观：在外部血测量 BLyS 和它的受体 mRNA 的表示层次用即时荧光的单音的原子房间(PBMC ) 量的聚合酶链反应(RFQ-PCR ) 方法并且调查在 BLyS 和它的 receptorsmRNA 表示和全身的豺狼座 erythematosus (SLE ) 之间的关系。方法：特定的教材和 TaqMan 探查被设计，并且 RFQ-PCR 被执行。根据原生质标志 DNA 的标准曲线，在有 SLE 和 23 个健康题目的 23 个病人的 BLyS 和它的受体 mRNA 表示的水平我们 redetermined。到β _ 2-microgluobulin (β _ 2M ) 的 BLyS mRNA 的拷贝数字的比率 mRNAand 到β _ 的 BLyS 受体 mRNA 的拷贝数字的比率 2 M mRNA 为 BLyS 和 BLyS mRNA 表示的层次被认为是指示物。结果：RFQ-PCR 的集中在 10 的范围 - 10 ~ 为 bothintra 试验性、内部试验性的重制度的变化价值的 9 pg/ml，和系数从 2.40% ～ 10.12% 并且从 4.26%to 12.29% 分别地。在 23 个 SLE 病人， BLyS 的水平和它的受体(BCMA， TACI， BAFF-R ) mRNA 在 1.27 ～ 1 .49， 0.64 ～ 0 .77， 0.83 ～ 1 .05 和 0.98 ～ 1 的范围 .37 分别地。吝啬的价值是 1.38 ± 0.07， 0.70 ± 0.04， 0.91 ± 0 .06 和 1.15 ± 0 .12 分别地。在 23healthy 施主， BLyS 和它的受体的层次(BCMA， TACI， BAFF-R ) mRNA 是：0.60 ～ 1 .0,0.55 ～ 0 .80， 0.54 ～ 0 .74 和 0.54 ～ 0 .77 分别地。吝啬的价值是 0.83 ± 0 .13， 0.68 ± 0 .08,0.65 ± 0.07 和 0.68 ± 0.06 分别地。结论：这结果建议那 BLyS， TACI andBAFF-R 可能涉及 SLE 和 mRNA 表示层次的致病可能为 SLE 的诊断被用作新标记。

英文摘要：

Objective： To measure the expression levels of BLyS and its receptors mRNA in peripheral blood ronnonuclear cells （PBMC） using real-time fluorescence quantitative polymerase chain reaction（RFQ-PCR） method and to investigate the relationship between BLyS and its receptors mRNA expression and systemic lupus erythematosus （SLE）. Methods： Specific primers and TaqMan probe were designed, and RFQ-PCR was perfomed. According to the standard curve of plasmid DNA, the level of BLyS and its receptors mRNA expression in 23 patients with SLE and 23 healthy subjects were detemined. The ratio of the copy number of BLyS mRNA to that of β2-microgluobulin （β2M） mRNA and the ratio of the copy number of BLyS receptors mRNA to that of β2M mRNA were regarded as indicator for the levels of BLyS and BLyS mRNA expression. Results： The concentration of RFQ-PCR was in the range of 10 - 109 pg/ml, and the coefficient of variation values for both intra-experimental and inter-experimental reproducibility ranged from 2.40% to 10.12% and from 4,.26% to 12.29%, respectively. In 23 SLE patients, the level of BLyS and its receptors（BCMA, TACI, BAFF-R） mRNA were in the ranges of 1.27 ± 1.4,9, 0.64 ± 0.77, 0.83 ± 1.05 and 0.9±3 -1.37, respectively. The mean values were 1.38 ± 0.07, 0.70 ± 0.04, 0.91 ±0.06 and 1.15±0.12, respectively. In 23 healthy donors, the levels of BLyS and its receptors（BCMA, TACI, BAFF-R） mRNA were： 0.60±1.0, 0.55±0.80, 0.54±0.74 and 0.54± 0.77, respectively. The mean values were 0.83 ±0.13, 0.68±0.08, 0.65± 0.07 and 0.68 ± 0.06, respectively. Conclusion： This results suggest that BLyS, TACI and BAFF-R might be involved in the pathogenesis of SLE and the mRNA expression levels might be used as new markers for the diagnosis of SLE.