中文摘要：

目的：研究建立实时荧光定量（RFQ）-PCR法测定B淋巴细胞激活因子（BLyS）及其受体mRNA含量的方法，探讨其在系统性红斑狼疮（SEE）患者外周血单一核细胞（PBMC）中表达的检测价值。方法：在BLys及其受体基因保守区设计特异性的引物和荧光探针，用反转录（RT）-PCR扩增目的片段实时检测产物的荧光强度．用所建立的RFQ-PCR方法检测23例SLE患者、23名正常人外周血BLyS及其受体mRNA表达。结果：SLE患者B淋巴细胞成熟抗原（BCMA）、穿膜蛋白活化物（TACI）和B淋巴细胞活化因子受体（BAFF）-R mRNA的表达明显高于正常人（P〈0．01），活动期患者的表达高于缓解期（P〈0．01），抗ds-DNA抗体阳性和尿蛋白阳性的SLE患者BCMA、TACI和BAFF-RmRNA的表达高于阴性患者（P〈0.01）。结论：RFQ-PCR检测BLys及其受体mRNA含量的方法具有较好的灵敏度和重复性；SLE患者BLyS、TACI和BAFF—RmRNA表达含量升高，提示BLyS、TACI和BAFF-R可能与SLE的发病有关。

英文摘要：

Objective： To detect the expression of B lymphocyte stimulator （BLyS） and its receptor in peripheral blood mononuclear cells （PBMC） in the patients with systemic lupus erythematosus （SLE）. Methods： The primers and probe were designed which were specific for the mRNA of BLyS. Then the mRNA expressions of BLyS and its receptor in PBMC were detected respectively in 23 patients with SLE and 23 controls （healthy subjects） by using real-time fluorescence quantitative polymerase chain reaction （RFQ-PCR）. Results： The mRNA expressions of BCMA, TACI and BAFF-R in PBMCs were significantly higher in the patients with SLE than those in healthy controls （P〈0.01）. Further study showed that within the SLE patients the mRNA expressions were also higher in the patients with active SLE than those with inactive SLE （P〈0.01）. The mRNA expressions were also elevated in the patients with positive anti-dsDNA antibody and proteinuria （P〈0.01）. Conclusions： This assay is sensitive, reproducible and suitable for clinical practice. The mRNA expressions of BLyS, TACI and BAFF-R were significantly elevated in SLE patients, which suggests that BLyS, TACI and BAFF-R might be involved in the pathogenesis of SLE.