中文摘要：

目的探讨胰岛素样生长因子Ⅱm RNA结合蛋白3（IMP3）、Ki-67、P16INK4 a在宫颈上皮内瘤变（CIN）及浸润性癌中的表达与临床病理意义。方法采用免疫组化S-P二步法及原位杂交方法检测20例正常宫颈上皮（NCE）、100例CIN、80例宫颈鳞状细胞癌（SCC）患者的IMP3蛋白、Ki-67及P16INK4 a蛋白的表达情况。结果IMP3蛋白在NCE、CIN（Ⅰ、Ⅱ、Ⅲ）、SCC中的阳性表达率分别为0.0%、0.0%、40.0%、71.1%、91.2%,两两比较差异均有统计学意义（P〈0.05）,IMP3蛋白表达与宫颈病变程度呈正相关。P16INK4 a蛋白在NCE、CIN（Ⅰ、Ⅱ、Ⅲ）和SCC中的阳性表达率分别为0.0%、52.0%、96.6%、95.5%、97.8%。SCC中IMP3及P16INK4 a的阳性表达率均与组织学分化有关（P〈0.05）,IMP3蛋白与P16INK4 a蛋白的表达呈正相关（r=0.390,P=0.005）;Ki-67在SCC以及CIN中广泛表达,少数正常子宫颈组织中也有表达,在正常组、CIN组以及SCC组中的阳性表达率不同,其差异有统计学意义（P〈0.05）。结论IMP3、Ki-67及P16INK4 a表达可为早期宫颈癌的病理诊断提供一定的实验依据,可将其作为宫颈癌转移潜能的生物学标记物之一。

英文摘要：

Objective To explore the expressions and clinical significance of insulin-like growth factor Ⅱ m RNA-binding protein3（ IMP3）,Ki-67,and P16INK4 ain cervical intraepithelial neoplasia（ CIN） and invasive cervical cancer. Methods Immunohistochemical SP two-step method and in situ hybridization were used to detect the expressions of IMP3,Ki-67,and P16INK4 ain 20 cases with normal cervical epithelium,100 cases with CIN,and 80 cases with cervical squamous cell carcinoma. Results The positive expression rates of IMP3 in normal cervical epithelium,CINⅠ,CINⅡ,CINⅢ,and cervical squamous cell carcinoma were 0. 0%,0. 0%,40. 0%,71. 1%,and91. 2%,respectively,significant differences were observed by pairwise comparison（ P〈0. 05）. IMP3 expression was positively correlated with the degree of cervical lesions. The positive expression rates of P16INK4 ain normal cervical epithelium,CINⅠ,CINⅡ,CINⅢ,and cervical squamous cell carcinoma were 0. 0%,52. 0%,96. 6%,95. 5%,and 97. 8%,respectively; the positive expression rates of IMP3 and P16INK4 ain cervical squamous cell carcinoma were correlated with histological differentiation（ P〈0. 05）,there was a positive correlation between IMP3 expression and P16INK4aexpression（ r = 0. 390,P = 0. 005）; Ki- 67 expressed in cervical squamous cell carcinoma and CIN widely,and it expressed in few normal cervical tissues,there was statistically significant difference in the positive expression rate of Ki-67 among normal cervical epithelium,CIN,and cervical squamous cell carcinoma（ P〈0. 05）. Conclusion The expressions of IMP3,Ki-67,and P16INK4 acan provide certain experimental basis for pathological diagnosis of early cervical cancer,which can be used as biomarkers of metastatic potentiality of cervical cancer.