中文摘要：

摘要：目的研究2-羟基-1-甲氧基阿朴啡在Beagle犬体内的代谢行为。方法选择健康Beagle犬3只单剂量1．5×10-3g·kg-1灌胃2-羟基-1-甲氧基阿朴啡，于给药后60rain前肢静脉取血，3000r．miff’离心10min，分取血浆，将血浆经SPE—C18固相柱纯化后，采用LC—MS^n方法对血浆中待测代谢产物进行总离子监测（TIM）和多级全扫描质谱分析（MS^n）。结果在Beagle犬血浆中检测到母体成分2-羟基-1-甲氧基阿朴啡和3种代谢产物。结论2-羟基-1-甲氧基阿朴啡在Beagle犬体内主要与葡萄糖醛酸相结合形成II相代谢产物。

英文摘要：

OBJECTIVE To investigate the major metabolites of 2-hydroxy-1-methoxyaporphine in Beagle dogs. METHODS The blood samples were collected from vein after a single dose of 1.5 mg.kg-1 2-hydroxy-1-methoxyaporphine. The plasma samples were purified by a SPE column （SPE-CIs） and analyzed with LC/MSn for potential metabolites. RESULTS 2-hydroxy-l-methoxyaporphine and its three metabolites were first identified in Beagle dogs plasma. CONCLUSION The major metabolic pathway of 2-hydroxy-1-methoxyaporphine in Beagle dogs is conjugating with glucuronic acid, forming phase II mctabolites.