中文摘要：

目的研究鉴定2，3，5，4′-四羟基二苯乙烯-2-D-β-D-葡萄糖苷（SBGC）在Beagle犬体内的代谢行为。方法选择健康Beagle犬3只单剂量0.4g·kg^-1灌胃SBGC，于给药后60min后肢静脉取血，3000r·min^-1离心10min，分取血浆，将血浆经SPE—C18固相柱纯化后，采用LC／MSn方法对血浆中的待测的代谢产物进行总离子监测（TIM）和多级全扫描质谱分析（MSn）。结果在Beagle犬血浆中检测到母体成分SBGC和4种代谢产物。结论SBGC在Beagle犬体内主要与葡萄糖醛酸相结合形成Ⅱ相代谢产物。

英文摘要：

OBJECTIVE To investigate the major metabolites of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D- glucoside in Beagle dogs. METHODS The blood samples were collected from vein after a single dose of 0.4 mg·kg^-1 2,3,5,4′-tetrahydroxystilbene-2- O-β-D-glueoside. The plasma samples which were separated after eentrifugation （3 000 r ·min^-1 ） were purified by a SPE column （Sep-C18） and analyzed with LC/MSn for potential metabolites. RESULTS 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside and its four metabolites were first identified in Beagle dogs plasma. CONCLUSION The major metabolic pathway of 2,3,5,4′tetrahydroxys- tilbene-2-O-β-D-glucoside in Beagle dogs is conjugating with glucuronic acid, forming phase Ⅱ metabolites.