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中文摘要：
为了分析江苏省流行的猪繁殖与呼吸综合征病毒（PRRSV）的遗传变异特征,采用RT-PCR方法对江苏地区分离的PRRSV NJGC株的ORF5基因进行了克隆和测序,利用DNAstar软件与国内外代表性毒株进行了同源性分析与序列对比,并绘制了系统进化树。结果表明：NJGC株属于美洲型PRRSV,其ORF5基因与美洲型代表毒株VR-2332的同源性为90.5%,与中国的经典毒株CH-1a的同源性为92.5%,与中国高致病性PRRSV毒株的同源性在98.0%以上;进化树分析结果显示,NJGC株和高致病性PRRSV在同一进化分支,推测其属于高致病性PRRSV,且与2006年分离的SY0608有更近的亲缘关系,体现了PRRSV具有一定的地域性。同时,该基因编码蛋白的抗原表位和抗原性也发生了变化。依据序列推断PRRSV NJGC株属于具有明显地域性的高致病性PRRSV。
英文摘要：
To analyze the genetic variation of porcine reproductive and respiratory syndrome virus（PRRSV） in Jiangsu Province,the ORF5 genes of PRRSV strain NJGC were amplified,cloned and sequenced by RT-PCR,and blasted with the representative isolates using DNAstar software.The results showed that PRRSV isolate NJGC belonged to the North American genotype.The ORF5 gene of strain NJGC shared 90.5% sequence similarity with the North American prototype VR-2332,92.5% similarity with CH-1a,the first PRRSV isolate in mainland China in 1996,and over 98.0% similarity with the highly pathogenic PRRSV.The phylogenetic tree indicated that the PRRSV NJGC possed a same branch with the highly pathogenic PRRSV,and shared a closer relationship with the SY0608.The typical geographic genetic relationship existed in the Jiangsu strain of PRRSV.Meanwhile,there are changes in the epitopes and the antigenicity of ORF5 encoded protein.
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