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中文摘要：
根据已发表的其他脊椎动物β-珠蛋白基因序列设计并合成1对特异性引物，通过RT-PCR方法首次克隆到美国红鱼和大黄鱼β-珠蛋白cDNA，其开放读码框均由447个碱基组成，编码148个氨基酸的多肽，预测的相对分子量分别为16333和16148Da，两者核苷酸序列和推导的氨基酸序列同源性分别为90．4％与81．2％。将推导的美国红鱼和大黄鱼β-珠蛋白氨基酸序列与其他鱼类及人β-珠蛋白氨基酸序列进行同源性比较，其同源性在35.5％～70．3％，它们与同属真口鱼纲的真鲷、黄尾鲱鱼、鲤鱼、虹鳟等的同源性较高，而与肉鳍鱼纲的肺鱼及板鳃纲的鲨鱼的同源性较低；二级结构预测表明，美国红鱼和大黄鱼β-珠蛋白存在类似哺乳动物的8个α-螺旋区，在近端与远端与血红素结合的组氨酸高度保守。
英文摘要：
The β-globin gene and amino acid sequence of large yellow croaker （ Pseudosciaena crocea ） and red drum（ Sciaenops ocellatus ）were first described. The entire open reading frames of two β-gjobin genes were beth 447 bp long and codified 148 amino acid residues, The homology between them was 90.4 % for nucleotides and 81.2 % for deduced amino acids.Amino acid identity of two β-globin genes compared with those reported in other vertebrates including shark, teleosts and human, ranged from 35.5 to 70,3 %, The proximal histidine residues at the heme binding region and the important functional residues that were involved in the contacts with the heme and between interfaces were conserved.
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