中文摘要：

该文的目的是测量PCR温度的精确性和PCR仪上孔间以及不同循环和不同次测量的温度均一性。PCR程序：95℃（变性），30s；55℃（退火），40s；72℃（延伸），50s；6个循环。孔和管内的温度重复性都很好；变性阶段，孔和管内液体温度与程序温度偏差较大，孔及管内的温度均一性和重复性均很好；孔内温度不能达到通常所需的温度（90℃以上）的比例为22．2％。退火和延伸阶段孔和管内的温差较小；有2个孔不能达到通常需要的温度。实际温度在各阶段停留的时间分别占设定时间的90％的比例较低。这些缺陷可能导致扩增结果假阳性或假阴性。

英文摘要：

Both temperature accuracy of wells at block and uniformity of well-to-well were tested on a standard PCR process. The primary thermal cycling settings were 6 cycles of 95℃ （denaturation） for 30 s, 55 ℃ （annealing） for 40 s, 72 ℃ （extension） for 50 s, The results are the temperatures of wells and liquids in mierotubes measured have good repeatability cycle-to-cycle respectively. Temperatures of wells have poor uniformity at denaturation step and deviated far from the programmed temperature. During denaturation, temperatures of 22.2% wells cannot reach the required denatured temperature （above 91 ℃）, while during annea- ling and extension temperatures, 2 wells cannot reach the required reaction needed temperatures （ annealing at 55 ℃ to 56℃, extension at 70 ℃ to 72 ℃ ） respectively. Percentages of holding time at above three steps temperatures of 90% programmed times are very low. The conclusion is false positive or false negative may be induced because of these shortcomings.