中文摘要：

采用PCR-SSCP方法检测猪激素敏感脂肪酶（hormone sensitive lipase，HSL）基因外显子1的多态性，并分析其与初生重、断奶重、6月龄重和背膘厚的关联性。根据猪HSL基因的DNA序列（AJ000483）设计5对引物，结果在P5引物对扩增的片段上发现了多态性，并对纯合子进行测序，发现外显子1的874bp处存在G-A转换，且存在3种基因型（AA、AB、BB）。统计结果表明，3种基因型在各品种中的分布不一致，长白猪和大白猪与莱芜猪和沂蒙黑猪比较差异极显著（P〈0．01），长白猪与大白猪比较，莱芜猪与沂蒙黑猪比较差异均不显著（P〉0．05）。固定效应模型分析结果表明，背膘厚基因型间差异显著（P〈0．05），而初生重、断奶重和6月龄重基因型间差异均不显著（P〉0．05）。最小二乘分析结果表明，BB基因型个体同AB和AA基因型个体比较背膘厚的差异显著（P〈0．05），3种基因型在背膘厚的大小排列顺序为BB〈AB〈AA。因此，推测HSL基因对个体胴体瘦肉率存在一定的影响将HSL某因应用于猪育种过程中的标记辅助选择可以加快猪的育种进程。

英文摘要：

Single-nucleotide polymorphisms of exonl of hormone sensitive lipase （HSL） gene were tested using PCR-SSCP. Correlations between the HSL gene and the birth weight, the weaning weight, the weight at the six-month and the backfat thickness were analyzed. On the basis of the published DNA sequence （AJ000483） of the porcine HSL gene, five pairs of primers were designed, and one polymorphism was found in the PCR product amplified with P5 primers. A G→ A transition at 874 site of exonl of HSL gene was detected by sequencing the homozygotes. Three genotypes existed. The results show that： firstly, the genotype distribution wasn＇t in consistent among the different pig breeds. Significant differences （P〈0.01） were found for the genotype distribution while the Landrace and the Large White compared with the Laiwu and the Yimeng pig breeds; whereas no significant differences （P〉0.05） existed while the Landrace compared with the Large White, the Laiwu compared with the Yimeng pig breeds. Secondly, on the basis of the fixed effect model, significant differences （P〈0.05） were found in the backfat thickness, whereas no significant differences （P〉0.05） existed in the birth weight, the weaning weight and the weight at the six-month. Thirdly, using least square analysis, it was shown that individuals of the BB genotype have significantly lower （P〈0.05） backfat thickness than those of AB and AA genotypes, with the order being BB〈AB 〈AA. These results suggest that HSL gene has significant effects on fat deposit. The HSL gene can be used in marker-assisted selection to accelerate the breeding progress.