中文摘要：

目的：研究大豆蛋白酶抑制剂对内毒素致大鼠急性肺损伤的防治作用。方法：SD雄性大鼠50只，分为假手术组，模型组，阳性药苯甲磺酰氟（PMSF）组，大豆蛋白酶抑制剂高、中剂量组共5组，每组10只。除假手术组外其余各组大鼠气管内注入内毒素（6mg·kg^-1），假手术组大鼠气管内注入等量的生理盐水。造模后第1天腹腔注射治疗药物，大豆蛋白酶抑制剂（SBTI）高、中剂量组分别150，100mg·kg^-1·d^-1。阳性药组大鼠给与等体积的苯甲磺酰氟50mg·kg^-1·d^-1；假手术组和模型组大鼠腹腔注射等量的生理盐水。造模后第7天取材，测定肺系数、肺通透指数、肺泡灌洗液中细胞计数和中性粒细胞百分比、弹性蛋白酶样活性，肺组织NF-κB和IκBa的活性测定，HE染色进行病理学观察。结果：PMSF和SBTI均能降低急性肺损伤大鼠肺系数、肺通透指数，降低肺泡灌洗液中的细胞总数、活化中性粒细胞百分比和弹性蛋白酶样活性。其中以阳性药组和SBTI高剂量组疗效较显著，阳性药疗效优于SBTI高剂量组。SBTI组大鼠肺组织核蛋白中NF-κB p65的含量明显降低（P〈0．01），SBTI组大鼠肺组织胞浆蛋白中IκBα的含量明显升高（P〈0．05）。结论：大豆蛋白酶抑制剂显著降低肺组织的炎症反应，减轻肺组织损伤，对内毒素致大鼠肺损伤具有一定的保护作用。

英文摘要：

Objective： To study the protective effect of soybean protease inhibitor on LPS-induced lung injury in rats. Method： Fivty male SD rats were randomly devided in five groups, 10 rats in each group as sham-operation group, model control group, positive medicine group, and high, moderate SBTI groups. Except the sham-group, other groups were induced by intratracheal instilla- tion of LPS with a dose of 6 mg · kg^-1. All rats were given drug throught intraperitoneal injection except the model controlled group, the positive medicine group was given PMSF with a dose of 50 mg · kg^-1 , the high dose group of SBTI was given SBTI with a dose of 100 mg · kg^-1 , a dose of the moderate group is 50 mg · kg^-1. We examed all rats in seven days. Index exam： cell quantity, activity of neutrophilic granulocyte released elastic protease proteins in BALF, histopathological examination and so on. Result： Soybean protease inhibitor can level down the level of total protein, cell quantity, PMN percent, activity of neutrophilic granulocyte in BALF. SBTI level down the content of NF-κB in nucleoprotein, while increase the content of IκBα in plasmoprotein. Conclusion： SBTI is useful in protecting experimental pulmonary injury induced by LPS in rats.