从污染鱼塘底泥中筛选分离出一株能有效降解低浓度呋喃唑酮的细菌F5,经16S rDNA同源性序列分析,鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。30℃静置避光培养30d后,该菌株对0.75、1.0和2.5mg/L呋喃唑酮的降解率分别为58.8%、64.1%和38.2%。温度为20~25℃更有利于该菌株的降解作用,对1.0mg/L呋喃唑酮降解率均能达85%。碳源和氮源浓度的提高均能促进该菌株的生长和对呋喃唑酮的降解。在0.5mg/L低磷浓度下,该菌株生长受抑制但降解活性增加,对1.0mg/L呋喃唑酮降解率达71.8%。
A Strain of bacterium F5 with high furazolidone degradation was selected from the polluted sediment of a fish pond after tamed and enriched. Through sequence analysis of the 16S rDNA, F5 was identified as Pseudomonas aeruginosa. When F5 was incubated in enrichment liquid medium which contained 0. 75, 1.0, 2. 5 mg/L furazolidone respectively, the degradation rates were 58.8% , 64. 1% and 38.2% respectively after 30 d at 30 ℃. The optimum temperature for biodegradation was 20 -25 ℃ and the degradation rate for 1.0 mg/L furazolidone was about 85 %. Glucose and nitrogen can improve furazolidone degradation. Phosphorous can inhibit the growth of FS, but the activity of strain was improved, and the degradation rate was 71.8%.