以青霉菌(Penicillium sp.)为吸附剂,对水溶液中染料中性红和孔雀绿进行了吸附研究.试验考察了预处理方法、pH值和重金属离子对吸附的影响,并对吸附机理进行了探讨.结果表明,在试验选用的预处理剂中,碳酸氢钠预处理的菌体吸附效果最好;溶液的pH值为5—6时,对染料的吸附量达到最大.当溶液中含有50mg·l^-1 Pb^2+,Zn^2+,Cu^2+任一种金属离子时,对菌体吸附中性红和孔雀绿均有抑制作用.碳酸氢钠处理菌体对中性红和孔雀绿的吸附过程可用准二级动力学模型来描述,相关系数均为0.9997.菌体吸附中性红和孔雀绿过程中有Na^+,K^+和Mg^2+的释放,表明吸附过程中存在离子交换吸附机制.此外,吸附过程中静电吸附也起到一定的作用.
The biosorption of neutral red and malachite green in aqueous solution with Penicillium sp. as well as the influence of pretreatment methods, pH and heavy metals on adsorption was investigated. The adsorption mechanism of dyes was also revealed. Among several pretreated biomass preparations, NaHCO3-treated biomass was the best one and obtained the maximum uptakes of dyes at pH 5-6. At the present of any heavy metal ion of Pb^2+ , Cu^2+ or Zn^2+ of 50mg·l^-1in aqueous solution, the biosorption capacity of neutral red and malachite green was decreased. The adsorption kinetics of dyes on NaHCO3-treated mycelia could be described by the pseudo second-order reaction model, and the correlation coefficients were both 0. 9997. NaHCOa-treated mycelia released Na^+ , K^+ and Mg^2+ after adsorption, The result showed that ion'exchange was important mechanism in biosorption of neutral red and malachite green. Electrostatic interaction mechanism could also play a role in the biosorption of dyes on pretreated biomass.