用草分枝杆菌作吸附剂,探讨了采用生物吸附-浮选法去除水中Pb^2+的可能性,并对吸附剂与捕收剂的作用机理进行了分析.草分枝杆菌的浮选试验结果表明。阳离子型捕收剂对草分枝杆菌的捕收能力明显比阴离子型捕收剂的强.草分枝杆菌的吸附-浮选试验结果表明,浮选过程可在10min左右达到终点,pH在4~7时,阳离子型捕收剂对草分枝杆菌有较好的浮选去除率,Pb^2+去除率也很高.当二正丁胺的浓度为45mmol/L,pH=4.75时,草分枝杆菌和Pb^2+的去除率分别达到92%和98%.动电位测试表明,草分枝杆菌的等电点为pH=3.09;吸附Pb^2+或与二正丁胺发生作用后,草分枝杆菌的等电点均有所增加.草分枝杆菌细胞壁表面具有较强的负电性,使得阳离子捕收剂对其具有较强的捕收能力,这对采用生物吸附-浮选法去除废水中的重金属离子可能会有重要意义.
The possibility of removal of heavy metals from waste water by adsorption flotation using Mycobacterlum phlei as adsorbent was investigated, and the collection mechanism of collectors on adsorbent was analyzed. From the single flotation tests, it shows that cationic collectors have a stronger collecting ability for Mycobacterium phlei than anionic collectors. The adsorptive flotation experiment shows that floatability process occurred within 10 minutes, the recovery of Mycobacterium phlei and the removing rate of Pb^2+ are high by using cationic collectors during pH value from 4 to 7. At 45mmol/L of Di-buty lamine as collector, and 4.75 of pH, the recovery of Mycobacterium phlei and the removing rate of Pb^2+ are 92 % and 98 % The isoelectric point of Mycobacterium phlei is 3.09 at pH of the solution, which increased when Pb^2+ or Di-buty lamine is adsorption by M:ycobacteriurn phlei, The good floatability of Mycobacterlum phlei with cationic collectors results from the intense zeta potential on the surface of cell, Adsorptive flotation may have practical applications for the removal of hazardous metals from contaminated water supplies.