通过扫描电子显微镜、能量分析谱、红外光谱和悬浮酵母菌的动电电位等分析手段,研究了悬浮酵母菌对Hg(Ⅱ)的吸附机理。由扫描电镜图像及能量分析谱可以看出,悬浮酵母菌吸附Hg(Ⅱ)后形态发生变化,悬浮酵母菌对Hg(Ⅱ)的吸附存在离子交换吸附;动电电位及pH曲线分析表明,悬浮酵母菌的零电点为pH=3.7,pH值是影响吸附效果的重要因素,溶液pH值为4.33时,吸附效果最好,去除率为99.95%;溶液pH〈4.0时,静电作用为主要的吸附机理;从吸附等温线可以得出,在溶液中Hg(Ⅱ)的初始质量浓度为601.8mg/L,菌质量浓度为20g/T.时,吸附达到饱和,此时的负载量为1024mg/g;红外光潜分析表明,悬浮酵母菌剥Hg(Ⅱ)的主要吸附位点为-OH、-NH,C=0、P=0和S=0。
The present paper is to introduce the authors' research into the mechanism of Hg(Ⅱ) biosorption by saccharomyces eerevisiae while applying modern analysis means, such as SME, electron spectrum, FT-IR analysis, and zeta potential apparatus. The contrasted resuhs of SME and electron spectrum show that the form of saccharomyces cerevisiae has changed as the result of the biosorption on Hg^2+ . The other findings of our research can be stated as follows. (l) An obvious hydronium commutation in the course of saccharomyces cerevisiae biosorption on Hg^2+ ; (2) The isoelectric point of saceharomyces cerevisiae proves at pH = 3.7 as the result of zeta potential and pH curve's analysis; (3) As an important affecting factor, the pH value is 4.33 ; (4) When the value of pH is under 4.33, i.e. on the ideal adsorbing condition, the removal rate can reach 99.95%, with the biosorption of Hg( Ⅱ ) by saccharomyces cerevisiae being the main static biosorption. From the absorbing isotherm, it can be found that, when the initial concentration of Hg solution is 601.8 mg/L, the concentration of the germ will be 20 g/L, with the biosorption reaching saturation, and the loading quantity will be 10.24 mg/g. Thus, the FF-IR analyses indicate that the mainly adsorbing positions of saccharomyces cerevisiae to Hg( Ⅱ ) are --OH, --NH2, C = O, P = O, and S = O.