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接种根瘤菌对紫花苜蓿吸收转运PCB28及抗PCB28污染能力的影响

ISSN号：1004-3268
期刊名称：《河南农业科学》
时间：0
分类：S551.7[农业科学—作物学]
作者机构：[1]丽江师范高等专科学校,云南丽江674100, [2]中国科学院南京土壤研究所土壤环境与污染修复重点实验室,江苏南京210008, [3]中国科学院烟台海岸带研究所,山东烟台264003
相关基金：国家自然科学基金重点项目（40621001,40701080）;中国科学院知识创新工程项目（KZCX2-YW-404,CXTD-Z2005-4）

关键词：多氯联苯, 紫花苜蓿, 根瘤菌, 吸收转运, polychlorinated biphenyls, alfalfa, rhizobia, absorption increased in alfalfa,and thus anced. and transportation

中文摘要：

为探讨豆科植物一根瘤茵共生体系对多氯联苯（PCBs）污染土壤的生物修复机制，以紫花苜蓿作为供试植物，研究了PCB28（2，4，4＇-三氯联苯）胁迫下接种根瘤菌（野生型、突变株）对紫花苜蓿吸收转运PCB28及抗PCB28污染能力的影响。结果表明，PCB28胁迫下，接种根瘤菌可提高紫花苜蓿各部位对PCB28的吸收富集能力，其中4mg／LPCB28处理条件下，与不接种根瘤茵处理（P）相比，接种野生型根瘤茵处理（P＋R）和接种根瘤菌突变株处理（P＋SMY）紫花苜蓿各部位PCB28含量显著增加，其中茎叶PCB28含量分别增加140．9％和91．6％；根PCB28含量分别增加64．0％和44．4％；根瘤对PCB28富集能力最高，分别达1362．10ptg／kg和1111．34μg／kg。PCB28胁迫下，接种根瘤菌可增强紫花苜蓿的固氮能力，促进其生长，提高其各部位生物量，不同处理条件下紫花苜蓿根、茎叶全氮含量均表现为P＋R〉P＋SMY〉P，生物量表现出相同的变化趋势。在PCB28胁迫下接种根瘤菌尤其是野生型根瘤菌，可显著增加紫花苜蓿体内过氧化物酶（POD）、超氧化物歧化酶（SOD）活性，从而提高紫花苜蓿对PCBs的抗逆性，促进PCBs在紫花苜蓿体内的代谢转化。

英文摘要：

In order to explore the phytoremediation mechanism of Leguminosae-rhizobia symbiosis in poIychloriated biphenyls（PCBs） contaminated soil,alfalfa（Medicago sativa L. ） was used as the experimental plant,and wild type and mutative rhizobial strains were used to investigate the effects of rhizobia on absorption, transportation of PCB28 and PCB28 tolerance of alfalfa under PCB28 stress. The results showed that the absorption and transportation ability to PCB2 was increased when alfalfa was inoculated with rhizobia. Compared with the alfalfa without rhizobia（P）,when the alfalfa was inoculated with wild type rhizobial strains（P＋R） and mutative rhizobial strains（P＋SMY） ,the concentration of PCB28 was significantly increased by 140.9% and 91.6% in shoots,64.0% and 44.4% in roots,the concentration of PCB28 was highest in root nodules, about 1 362.10 μg/kg and 1 111.34μg/kg respectively. The nitrogen content order in roots and shoots of alfalfa was P＋R〉P＋SMY）P, the biomass of shoots and roots showed the same trend. In addition,when alfalfa was inoculated with rhizobia especially wild type rhizobial strains, the activities of peroxidase（POD） and superoxide dismutase（SOD） the tolerance and transformation ability of alfalfa to PCBs were enhanced.

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