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中文摘要：
采用溶液摇瓶实验研究了苜蓿根瘤菌（Rhizobium meliloti）对三氯代联苯（2,4,4′-trichlorobiphenyl）单体以及18种多氯联苯（polychlorinated biphenyls,PCBs）混合物的降解转化能力.结果表明,接入苜蓿根瘤菌转化7d后,随着溶液中底物2,4,4′-TCB浓度的增加,该菌株对其的降解能力也逐渐提高,在1.0、5.0、10.0、25.0、50.0 mg·L^-1浓度条件下该菌对2,4,4′-TCB的降解率分别为34.0%、48.3%、69.7%、96.0%、98.5%,并且通过气相质谱分析,发现溶液中出现了一些新的代谢产物.同时,苜蓿根瘤菌对不同浓度下18种PCBs同系物混合物的降解能力,随着底物浓度的升高呈现出从低到高,然后降低,并趋于平衡的趋势,其最高降解率可达54.7%,并存在高氯代PCBs向低氯代PCBs的转化过程.
英文摘要：
The capability of Rhizobium meliloti on degrading 2,4,4′-trichlorobiphenyl and 18 kinds of polychlorinated biphenyl congener mixtures was studied by shaking flask experiment.The results showed that the degradation capability of Rhizobium meliloti to 2,4,4′-trichlorobiphenyl increased gradually with the increasing concentration of 2,4,4′-trichlorobiphenyl.After 7 days,the degradation rates of 2,4,4′-trichlorobiphenyl under 1.0,5.0 10.0,25.0,50.0 mg·L^-1 concentration of 2,4,4′-trichlorobiphrnyl were 34.0%,48.3%,69.7%,96.0%,98.5% respectively.Meanwhile some new intermediate products were found in the solution culture.The capability of Rhizobium meliloti on degrading 18 kinds of polychlorinated biphenyls congener mixtures appeared in a trend from low to high,then decreased to a certain balance with the increasing concentration of the mixture.The highest degradation rate was 54.7%,and moreover,a transformation process from higher chlorinated PCBs congeners to lower chlorinated PCBs congeners emerged.
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