中文摘要：

目的：研究导痰汤能否通过对核因子-κB（NF-κB）信号通路的调节而干预ICAM-1的表达,以揭示导痰汤治疗动脉粥样硬化的机制。方法：通过PCR和Western blot等方法,观察导痰汤对肿瘤坏因子-α（TNF-α）刺激脐静脉内皮细胞（HUVEC）培养内皮细胞细胞间黏附分子-1（ICAM-1）和NF-κB表达的影响。结果：TNF-α诱导组ICAM-1和NF-κB显著高于空白对照组和导痰汤对照组（P〈0.01）;使用导痰汤含药血清或吡咯烷二硫氧基甲酸（PDTC）处理后,ICAM-1和NF-κB p65表达显著下降（P〈0.05,P〈0.01）;NF-κB活性与ICAM-1 mRNA水平呈显著正相关（r=0.716,P〈0.01）。结论：导痰汤可以调节NF-κB信号通路,从而有效抑制TNF-α刺激所致的脐静脉内皮细胞ICAM-1的表达。

英文摘要：

objective： To observe the effect of daotan decoction to the expression of ICAM-1 and NF-κB in Human＇s Umbilical Veins Endothelial Cells.Methods： This study observed the effect of these cells＇ ICAM-1 and NF-κB expression,which was induced by TNF-α,by the way of PCR and Western blot methods.Results： Compared with two control groups,the expression of ICAM-1 and NF-κB in model group（TNF-α） was significantly increased（P0.01）.And compared with model group,Daotan Decoction could significantly restrain ICAM-1 and NF-κB expression（P0.05,P0.01）.Meanwhile,there was significant positive relationship between the expression of ICAM-1 and NF-κB（r=0.716,P0.01）.Conclusion： Daotan Decoction could restrain ICAM-1 expression stimulated by TNF-α in human＇s umbilical veins endothelial cells by regulating the NF-κB pathway.