中文摘要：

T 淋巴细胞被发现是包括 apoptosis 的高度对射线敏感、复杂的细胞的回答的人能在暴露之上被导致到 X 光检查照耀。然而，与照耀联系的 apoptosis 的机制不是清楚的。在这研究，一个 proteomic 方法在照耀以后在人的 T 淋巴细胞房间的 proteome 的改变上被用于调查。Jurkat 房间与 4 Gy X 光检查被照耀，房间 lysates 在在照耀(6， 12， 18， 24 和 48 h ) 以后的不同时间是镇定的。整个蛋白质被二维的荧光差别胶化电气泳动，然后差别分开并且确定表示蛋白质被集体 spectrometry 识别。4 蛋白质在丰富展出了重要导致照耀的差别，包括 L-plastin， bifunctional 嘌呤生合成蛋白质，导管素贝它链，贝它肌动朊。差别表示了蛋白质向被汇报直接或间接地涉及人的 T 淋巴细胞的功能。因此，这研究可能提供线索与与照耀有关的生物意义识别蛋白质。

英文摘要：

Human T lymphocytes were found to be highly radiosensitive and complex cellular responses including apoptosis could be induced upon exposure to X-ray irradiation. However, the mechanism of apoptosis associated with irradiation was not clear. In this study, a proteomic method was applied to investigation on alteration of proteome of human T-lymphocyte cells after irradiation. The Jurkat cells were irradiated with 4 Gy X-ray and the cell lysates were collected at different times after irradiation （6, 12, 18, 24 and 48 h）. The whole proteins were separated and quantified by two-dimensional fluorescence difference gel electrophoresis, and then the differentially expressed proteins were identified by mass spectrometry. 4 proteins exhibited significant irradiation-induced difference in abundance, including L-plastin, bifunctional purine biosynthesis protein, tubulin beta chain, beta-actin. Differentially expressed proteins were reported to be directly or indirectly involved in the function of human T lymphocyte. Thus, this study might provide clues to identify proteins with biological significance related to irradiation.