中文摘要：

目的：观察高糖环境下豚鼠膀胱Cajal样细胞形态学变化。方法：应用酶解法分离培养豚鼠膀胱Cajal样细胞,分为无糖组、正常对照组（葡萄糖浓度5mmol/L）、高糖组（葡萄糖浓度分别为15、30、60mmol/L）,通过光学倒置显微镜及c-kit抗体染色后激光扫描共聚焦显微镜观察细胞形态。结果：葡萄糖浓度分别为30、60mmol/L组较无糖组、正常对照组（5mmol/L）及15mmol/L组细胞数量减少,差异有统计学意义（P〈0.05）,蛋白标记后显示蛋白染色部位减少,有核着色迹象。结论：高糖环境可导致Cajal样细胞的形态异常,数量减少,可能引起其功能学的改变,提示其可能是糖尿病膀胱病变的的影响因素。

英文摘要：

Objective：To investigate the morphology of interstitial cells of Cajal-like in high glucose environment. Methods： The interstitial cells of Cajal-like were culture collagen enzymolysis separation. They were divided into three groups： no glucose, control （5mmol/L）, high glucose （15,30,60mmol/L）. The cell morphological was observed by optics inverted microscope and laser scanning confocal microscopy. Result： The number of cells in high glucose group （30,60mmol/L） decreased down obviously, the difference was significant （P〈0.05）. The position of protein labeling decreased down, and the nuclears labeled too. Conclusion： The high glucose environment induced the abnomal morphosis of interstitial cells of Cajal-like, decreased the cells number, changed its function, and may be the factor of diabetes.