中文摘要：

目的构建含有人SCL基因的pDC315-EGFP真核表达载体,为糖尿病膀胱病变（DCP）的治疗提供新思路。方法从含有人SCL基因的质粒中,采用PCR方法扩增SCL基因,将目的载体pDC315-EGFP进行酶切后交换,其产物转化细菌感受态细胞,对克隆产物进行菌落PCR鉴定,对阳性克隆产物进行测序和比对分析,比对正确的克隆即为构建成功的质粒。结果 PCR扩增的基因片段长度为1 036 bp,测序结果以及重组质粒pDC315-EGFP-SCL阳性克隆凝胶电泳鉴定均证明SCL基因成功克隆到真核表达载体中。结论成功构建了真核表达载体pDC315-EGFP-SCL,该载体有望用于DCP的治疗。

英文摘要：

Objective To construct pDC315-EGFP expression vector containing human SCL gene,so as to provide new clues for the treatment of diabetic cystopathy（DCP）.Methods PCR was performed to amplify SCL gene from the plasmid containing pur pose gene.Purpose vector pDC315-EGFP was cut with enzymes and exchanged.Produc t was translated into felling states cell of bacteria.The products in the bacter ial were identifed by PCR and positive products were analyzed by sequencing,pla smid containing correct SCL gene was successfully constructed.Results Length of specific fragment amplified was 1 036 bp.Sequencing results and correct bands by PCR identification proved that SCL gene have been cloned into pDC315-EGFP vec tor.Conclusion Eukaryotic expression vector can be successfully constructed,wh ich is expected to be used for the treatment of DCP.