中文摘要：

目的将在 adipocyte 区别和它行动的机制上调查钙隧道 blocker verapamil 的效果。从 3T3-L1 紧张老鼠胚胎的方法 Preadipocytes 进在 vitro 的成熟 adipocytes 有教养、区分。Verapamil 在 30 μ 的集中被加到文化媒介；白天 0 上的 mol/L。房间区别被 RT-PCR 被染色的油红 O 决定，标记基因 mRNA 表达式被计算并且比较。扫描共焦的显微镜学的 fluo-3/AM 探查和激光被用来测量细胞内部的钙集中。结果暴露于 verapamil 的 3T3-L1 preadipocytes 的区别率比未经治疗的房间的低。Verapamil 支持了 pref-1 基因表示的保留。在 verapamil 组的脂蛋白脂肪分解酵素表示在白天 4 ，白天 6 和白天 8 上的控制组是比那显著地低的( P < ； 0.05 )并且在表示抵抗在白天 6 上的控制组是比那显著地低的，白天 8 和白天 10 ( P < ； 0.05 )。在 verapamil 组的丰满的酸 synthase 表示在从白天 2 的控制组是比那显著地低的(P <； 0.05 ) 。钙的细胞内部的集中[在 verapamil 组的 Ca ²⁺]i 显著地在白天 2 ，白天 4 和白天 6 上在控制组与那些相比被减少( P < ； 0.05 )，当白天 0 上的二个组之间没有明显的差别时( P > ； 0.05 )。在 3T3-L1 preadipocytes verapamil 的结论显著地减少了 adipocyte 区别，下面调整为 adipocytes 区别的三标记基因的 mRNA 表示，并且延长了区别的一个禁止者的 mRNA 表示。区别上的 verapamil 的禁止的效果可以在 adipocytes 作为一块钙流入包含它的角色。

英文摘要：

Objective： To investigate the effect of the calcium channel blocker verapamil on adipocyte differentiation and its mechanism of action. Methods： Preadipocytes from 3T3-L1 strain mouse embryos were cultured and differentiated into matured adipocytes in vitro. Verapamil was added to the culture medium in the concentration of 30 μmol/L on Day 0. Cell differentiation was determined by Oil Red O staining and marker gene mRNA expression was evaluated and compared by RT-PCR. The fluo-3/AM probe and laser scanning confocal microscopy were used to measure intracetlular calcium concentrations. Results： （1）The differentiation rate of 3T3-L1 preadipocytes exposed to verapamil was lower than that of untreated cells. （2）Verapamil promoted the retention of pref-1 gene expression. Lipoprotein lipase expression in the verapamil group was significantly lower than that in the control group on Day 4, Day 6 and Day 8 （P 〈 0.05） and resistin expression was significantly lower than that in the control group on Day 6, Day 8 and Day 10 （P 〈 0.05）. Fatty acid synthase expression in the verapamil group was significantly lower than that in the control group from Day 2 （P 〈 0.05）. （3） Intracellular concentrations of calcium [Ca^2＋]i in the verapamil group were significantly decreased compared with those in the control group on Day 2, Day 4 and Day 6 （P 〈 0.05）, while there was no obvious difference between the two groups on Day 0 （P 〉 0.05）. Conclusion： In 3T3-L1 preadipocytes verapamil significantly reduced adipocyte differentiation, down-regulated the mRNA expression of three marker genes for adipocytes differentiation, and prolonged the mRNA expression of an inhibitor of differentiation. The inhibitory effect of verapamil on differentiation may involve its role as a blocker of calcium influx in adipocytes.