中文摘要：

以美洲南蛇藤幼嫩的顶芽或带腋芽的茎段为外植体,进行外植体消毒、增殖培养、瓶内生根和过渡移栽研究,结果表明：外植体用75%的乙醇消毒20 s,0.1%的HgCl2消毒2.0 min效果最佳;适宜的增殖培养基为MS＋6-BA 1.0mg·L^-1（单位下同）＋IBA 0.2＋3.0%蔗糖;壮苗培养基为MS＋6-BA 0.1＋IBA 0.05＋3%蔗糖;适宜的生根培养基为1/2MS＋IBA 0.5＋1.5%蔗糖,生根率82.0%;最佳过渡移栽基质为蛭石∶珍珠岩=2∶1,成活率为98.3%。

英文摘要：

Taking top buds and stems with axillary buds as the explants, the study of disinfection, proliferation culture, rooting in vitro and transition cultivating were conducted. The results were as follows： the most suitable disinfect time properly was 20s by alcohol and 2 min by HgCl2 respectively; proliferation culture media was MS＋6-BA1.0mg·L^-1 ＋ IBA0.2 ＋ Sucrose 3%; hardening-off culture media was MS＋6-BA0. 1 ＋ IBA0.05 ＋ Sucrose 3%; rooting culture media was 1/2MS＋IBA 0.5＋ Sucrose 1.5% and its rooting ratio was 82.0%; transition cultivating media was pearlite and peat,its rate was 2：1 ,survive ratio was 98.3 %.