中文摘要：

目的 18^F-FDG PET/CT显像评价肝癌H22模型及三萜类中药成分熊果酸（UA）对移植性肝癌（H22）细胞体内生长的抑制作用。方法昆明小鼠20只,雌雄各半,体重（20±2）g,右前腋皮下接种1×107/0.2 ml小鼠肝癌H22细胞,左前腋皮下接种等量生理盐水作为对照。建立实体瘤模型并进行PET/CT检查评价实体瘤模型后,随机分两组,对照组和熊果酸组（UA组）,每组10只,UA组每只按4.536 mg/kg给予体积0.2 ml的UA,每天1次,连续10 d;对照组给相同容积的生理盐水。给药结束后再次行18^F-FDG PET/CT显像。显像后处死小鼠,采用HE染色法检测形态学改变。结果 （1）20只小鼠治疗前右前腋皮下肿瘤接种部位放射性摄取/同侧肺（T右1/NT右1）为2.833±0.18,左前腋皮下接种等量生理盐水区放射性摄取/同侧肺（T左1/NT左1）为1.078±0.27,T右1/NT右1与T左1/NT左1有统计学差异（t=15.55,P=0.0000）。（2）对照组与UA组右前腋皮下接种肿瘤部位放射性摄取/同侧肺（T右2/NT右2与T右3/NT右3）分别为2.824±0.224及1.534±0.429,二者有统计学差异（t=11.85,P=0.0001）。左前腋皮下同等体积生理盐水接种放射性摄取/同侧肺（T左2/NT左2与T左3/NT左3）分别为1.078±0.33及1.076±0.34,二者无统计学差异（t=2.121,P=0.078）。结论18^F-FDG PET/CT显像是评价肝癌H22模型及熊果酸抑制肝癌效果的准确方法。

英文摘要：

Objective To evaluate model of HepA-ascitic type（H22）cell and the inhibiting effect of ursolic acid（UA）for H22 cell in vivo by 18F-FDG PET/CT imaging.Methods Kunming mice[n=20,10 males,10 females,（20±2）g]were translated by hypodermic（injection）with 1×107/0.2 ml H22 cell to right armpit and translated by hypodermic（injection）with same volume 0.9%NS to left armpit respectively and solid tumor model were established.It divided UA and control group after FDG PET/CT imaging,10 mice were in every group.About 0.2 ml UA（4.536 mg/kg）were pour into stomach of UA group from the first day to the tenth day.Control group were pour same volume 0.9%NS into stomach from the first day to the tenth day.In the eleventh day,the animals performed FDG PET/CT imaging again.Mice were executed after PET/CT imaging and the morphology was observed by HE.Results（1）Radioactivity uptake regions of translated tumor in right armpit vs.same side lung（TR1/NTR1）was 2.833±0.18,Radioactivity uptake regions of translated same volume 0.9%NS in left armpit vs.same side lung（TL1/NTL1）was 1.078±0.27 in 20 mice,TR1/NTR1 vs.TL1/NTL1 was significantly（P〈0.05）.（2）Radioactivity uptake regions of translated tumor in right armpit vs.same side lung（TR2/NTR2 vs.TR3/NTR3）by 18F-FDG PET/CT imaging were 2.824±0.224 and 1.534±0.429 in UA and control group respectively,between UA and control group had significant difference（P〈0.05）.Radioactivity uptake regions of translated same volume 0.9%NS in left armpit vs.same side lung（TL2/NTL2 vs.TL3/NTL3）were 1.078±0.33 and 1.076±0.34 in UA and control group respectively,between UA and control group had no significant difference（P〈0.05）.Conclusions 18F-FDG PET/CT imaging is an accurate and available method to evaluate model of HepA-ascitic type（H22）cell and the inhibiting effect of UA for H22 cell in vivo.