目的:应用HPCE对由山羊脾脏中获得的多肽进行分析。方法:由经前期诱导后山羊脾脏中提取多肽;经毛细管等电聚焦电泳(CIEF)的方法测定其组成及各组分的等电点。结果:毛细管等电聚焦电泳测得山羊脾脏多肽各组分等电点单一,约为pI=4.46。结论:由山羊脾脏中获得的多肽是由等电点单一的成分组成。
Objective:To analyze polypeptides from goat spleen by HPCE. Methods: Polypeptide was abstracted from goat spleen induced in advanced. The component and isoelectric point of all parts of the sample were determined by capillary isoelectric focusing (CIEF). Results:The result of CIEF shows that the polypeptide consists of the component with same isoelectric point, which is about 4. 46. Conclusion: The component of the polypeptide from goat spleen has same isoelectric point.