中文摘要：

(ZNF278 ) 锌手指蛋白质 278 是在人的纸巾是无所不在地分布式的新奇 Krueppel Cys2-His2-type 锌手指蛋白质。ZNF278 是否与 colorectal 癌症的发展有关，仍然是不清楚的。ZNF278 的 tran-scriptional 水平被即时聚合酶链反应在 colorectal 癌症学习。结果证明那 ZNF278 表情与相应非癌的纸巾相比在 53% colorectal 癌症纸巾被增加。ZNF278 的 Thetranscriptional 下面规定被检测在仅仅三(6%) 与相应非癌症纸巾相比的人的 colorectal 癌症纸巾。没有重要差别在 19 被检测(41%) 一些样品。然而，我们没能发现在抄写和年龄，性别，渗入的度，或肿瘤 colorectal 癌症缩放的 ZNF278 的起来规定之间的一个重要协会。在 colorectal carcinogenesis 学习 ZNF278 的函数，结肠癌房间线 SW1116 是稳定地有到构造的野类型的 ZNF278 plasmid 的 transfected 一个 overexpression 系统，并且短暂地有到构造的 ZNF278 的小介入 RNA 的 transfected 一个 ZNF278 击倒的系统。房间增长与 3- 被估计[4， 5-dimethylthiazol-2-yl ]-2,5-diphenyltetrazolium 溴化物染料和一个房间柜台。结果证明 ZNF278 支持房间生长，并且它的击倒压制房间增长。ZNF278 能是在 colorectal 癌症的潜在的 proto-oncogene。

英文摘要：

Zinc finger protein 278 （ZNF278） is a novel Krueppel Cys2- His2-type zinc finger protein that is ubiquitously distributed in human tissues. Whether ZNF278 is related to the development of colorectal cancer is still unclear. The transcriptional level of ZNF278 was studied in colorectal cancer by real-time polymerase chain reaction. The results showed that ZNF278 expression was increased in 53 % of colorectal cancer tissues compared to corresponding non-cancerous tissues. The transcriptional down-regulation of ZNF278 was detected in only three （6%） human colorectal cancer tissues compared to corresponding non-cancer tissues. No significant difference was detected in 19 （41%） pairs of samples. However, we failed to find a significant association between the up-regulation of ZNF278 transcription and age, sex, the degree of infiltration, or the tumor size of colorectal cancer. To study the function of ZNF278 in colorectal carcinogenesis, the colon cancer cell line SWlll6 was stably transfected with a wild-type ZNF278 plasmid to construct an overexpression system, and was transiently transfected with the small interfering RNA of ZNF278 to construct a ZNF278 knockdown system. Cell proliferation was assessed with 3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide dye and a cell counter. The results show that ZNF278 promotes cell growth, and its knockdown suppresses cell proliferation. ZNF278 could be a potential proto-oncogene in colorectal cancer.