中文摘要：

fluorometric 试金被用来学习摊开 Escherichia 关口 i RecQ helicase 导致的动力学的脱氧核糖核酸。这试金基于荧光回声精力转移并且在站流动，放松的脱氧核糖核酸被荧光黄源于催化 helicase 的脱氧核糖核酸放松的荧光排放改进在监视上执行了。由这个方法，我们决定了在不同的酶集中摊开 RecQ 的率的脱氧核糖核酸。我们也学习了在镁离子集中上摊开大小和率的脱氧核糖核酸的依赖。我们证明这个方法能被用来决定脱氧核糖核酸放松的极性。这试金应该极大地为催化 RecQ 的脱氧核糖核酸放松便于机制的进一步的学习。

英文摘要：

A fluorometric assay was used to study the DNA unwinding kinetics induced by Escherichia coli RecQ helicase. This assay was based on fluorescence resonance energy transfer and carried out on stopped-flow, in which DNA unwinding was monitored by fluorescence emission enhancement of fluorescein resulting from helicase-catalyzed DNA unwinding. By this method, we determined the DNA unwinding rate of RecQ at different enzyme concentrations. We also studied the dependences of DNA unwinding magnitude and rate on magnesium ion concentration. We showed that this method could be used to determine the polarity of DNA unwinding. This assay should greatly facilitate further study of the mechanism for RecQ- catalyzed DNA unwinding.