二价的离子的效果(Mn~(2+) , Mg~(2+) , Ca~(2+)) 在在直接存储器存取之间的相互作用上并且嘘一个人用荧光 anisotropy 试金被学习。荧光 anisotropiesof 直接存储器存取和 DNA-histone 面对二价的离子(Mn~(2+) , Mg~(2+) , Ca~(2+)) 被测量。结果显示那嘘当时,一个人减少 lambda 直接存储器存取的荧光 anisotropy 二价的离子(Mn~(2+),Mg~(2+),Ca~(2+))显著地提高荧光 anisotropy.Compared 到 DNA 的盒子独自孵化了 withhistone ,有更多嘘对 DNA 有约束力的什么时候二价的离子,嘘一个人和 DNA 一起被孵化。我们也发现 Mn~(2+) 更做 theDNA-histone 建筑群比另外的离子压缩了。
The effects of divalent ions (Mn^2+, Mg^2+, Ca^2+) on the interaction between DNA and histone are studied using a fluorescence anisotropy assay. Fluorescence anisotropies of DNA and DNA-histone in the presence of divalent ions (Mn^2+, Mgv, Ca^2+) are measured. The results indicate that histone reduces the fluorescence anisotropy of lambda DNA while the divalent ions (Mn^2+, Mg^2+, Ca^2+) significantly enhance the fluorescence anisotropy. Compared to the case of DNA incubated with histone alone, there are more histones binding to DNA when divalent ion, histone and DNA are incubated together. We also find that Mn^2+ makes the DNA-histone complexes more condensed than the other ions do.