中文摘要：

目的：观察并比较不同参数电针和不同温度艾灸对内脏高敏感模型大鼠穴区肥大细胞（mast cells,MC）活化的影响。方法：将50只内脏高敏感模型大鼠随机分组后分别给予1m A、3 m A电针和43℃、4℃艾灸刺激天枢穴,并与模型组和正常大鼠进行对照,采用甲苯胺蓝染色法观察各组大鼠天枢穴区肥大细胞M C数量、脱颗粒数、脱颗粒率情况,同时采用腹部撤回反射（abdominalwithdrawl reflex,AWR）评分评价各组大鼠的内脏高敏感反应。结果：与正常组和模型组比较,艾灸43℃组、艾灸4℃组、电针1m A组及电针3m A组大鼠穴区组织M C个数有显著性增加（P〈0.05,P〈0.01,P〈0.01,P〈0.01）,大鼠穴区组织M C脱颗粒数和脱颗粒率均有显著性增加（P〈0.01,P〈0.01,P〈0.05,P〈0.01）。与模型组相比,在20 mm Hg、40 mm Hg、0 mm Hg、80 mm Hg结直肠扩张（colorectal distension,CRD）刺激下,艾灸43℃组、艾灸4℃组、电针1m A组和电针3m A组的A WR评分均显著降低（20 mm Hg刺激下电针1 m A和电针3m A组,P〈0.05,其余均P〈0.01）;艾灸4℃组、艾灸43℃组大鼠在C RD刺激强度为20 mm Hg、40 mm Hg、0 mm Hg、80 mm Hg时与正常组的A WR评分均无统计学差异（均P〉0.05）;电针1m A组在0 mm Hg和80 mm Hg时AWR评分均显著高于正常组（均P〈0.01）;电针3m A组在0 mm Hg时AWR评分亦显著高于正常组（P〈0.01）,在20 mm Hg和80 mm Hg时亦高于正常组（均P〈0.05）;在40 mm Hg和80 mm Hg时,电针1m A组AWR评分高于艾灸4℃组（均P〈0.05）;电针3m A组在40 mm Hg时AWR评分高于艾灸4℃组（P〈0.05）。结论：不同参数电针和不同温度艾灸刺激对内脏高敏感模型大鼠穴区肥大细胞活化的影响以及改善其内脏高敏感反应存在差异,其中以4℃的艾灸效应最显著。

英文摘要：

Objective：To evaluate and compare electroacupunctures（EA） with different parameters and moxibustion at different temperatures influencing the activation of mast cells（MC） in Tianshu（ST 25） regions of visceral hyperalgesia model rats.Methods：Rats（except for model group） respectively accepted 1 m A or 3 m A EA or moxibustion at 43 or 4 to ℃ ℃stimulate Tianshu（ST 25） points after randomization of the fifty visceral hyperalgesia model rats,and then were compared with that in model and normal groups.Number,degranulation numbers,degranulation rates in Tianshu（ST 25） regions MC of rats in each group were observed using toluidine blue staining.Abdominal withdrawl reflex（AWR） score was used to evaluate the rat visceral hyperalgesia reactions.Results：Compared with the normal group and the model group,MC numbers（P〈0.05,P〈0.01,P〈0.01,P〈0.01）,degranulation numbers and degranulation rates（P〈0.01,P〈0.01,P〈0.05,P〈0.01） of Tianshu（ST 25） MC in regions tissues in 43 and 4 moxibustion groups,and 1 m A and 3 m℃ ℃A EA groups all increased significantly.Compared with the model group,AWR scores were significantly lower in 43 and 4 ℃ moxibustion groups,and 1 m A and 3 m℃A EA groups under the stimulation of 20 mm Hg,40 mm Hg,0 mm Hg or 80 mm Hg colorectal distension（CRD）（P〈0.05 in 1 m A and 3 m A EA groups under the stimulation of 20 mm Hg,P〈0.01 in the other groups）.AWR scores in 43 ℃and 4 ℃moxibustion groups under the stimulation of 20 mm Hg,40 mm Hg,0 mm Hg or 80 mm Hg CRD were not significantly different from those in the normal group（all P〈0.05）;AWR scores in 1 m A EA group under the stimulation of 0 mm Hg or 80 mm Hg were significantly higher than that in the normal group（P〈0.01）;AWR score in 3 m A EA group under the stimulation of 0 mm Hg was significantly higher than that in the normal group（P〈0.01）,and AWR scores in 3 m A EA group under the stimulation of 20 mm Hg or 80 mm Hg were also higher than that in the normal group?