中文摘要：

利用作者单位测得的和从GenBank检索得到的40株新城疫病毒（NDV）全基因组序列,采用SMS、DNA Star等生物信息分析软件,比较分析NDV全基因组的基本特征和分子进化规律。结果表明：40株NDV全基因组核酸序列长度有3种：15186、15192、15198nt,均符合6倍数规律;基因组A＋T含量占53%～54%,C＋G含量占46%左右;NDV基因组前三分之一（4500nt前）序列存在高GC含量区,特别是在大约1200～2400nt区域,GC含量最高达到69%;F基因片段构建的分子进化树显示,40株NDV划分为两大类（ClassⅠ,ClassⅡ）,基因组长度为15198nt的毒株属于ClassⅠ,其余的属于ClassⅡ;ClassⅡ毒株又分为基因Ⅰ～Ⅶ型,这些已测序的毒株中没有基因和型;病毒6个基因CDS序列构建的进化树显示,各基因进化基本保持一致,仅少数毒株存在差异,可能与病毒分子的基因重组有关。

英文摘要：

Forty complete genome sequences of Newcastle disease virus were obtained from GenBank or from our lab. The fundamental profiles and characteristics of molecular evolution were analyzed and compared through bioinformatics software programs-SMS and DNAStar. The results showed that all sequences presented three type of size in length, 15 186 nt, 15 192 nt, 15 198 nt, and all followed the rule of six. The AT content of genome was 53%-54%, whereas the GC content was around 46% respectively. The GC-rich was observed at the first one-third region of genome. Phylogenic analysis revealed that all sequences of NDV could be classified into two classes and seven known genotypes.