中文摘要：

在生理pH=7．4环境下，用摩尔比法测定γ-环糊精（γ-CD）与达旦黄（TY）的包合比（摩尔比）nγ-CD：^nTY=1：1，直线法测定包合常数所=495．23L／mol。以中性红（NR）作分子探针，用紫外光谱法、荧光光谱法、电化学法、化学热力学法和黏度法等研究包合物γ-CD-TY与鲱鱼精DNA的作用，得到γ-CD-TY包合物与鲱鱼精DNA作用的结合比nγ-CD-TY^nDNA=6：1，结合常数为K298.15K^⊙=1．12×10^5L／mol，K310.15K^⊙=2．72×10^5L／mol。热力学函数△rGm^⊙=-2．82×10^4J／mol，△rHm^⊙=5．67×10^4J／mol，△rSm^⊙=284．78J／（mol·K），说明γ-CD-TY包合物与DNA作用为熵驱动。确定γ-CD-TY与鲱鱼精DNA的作用方式为混合作用，同时存在部分嵌插和非嵌插作用方式。

英文摘要：

Under the condition of physiological environment pH 7.4, the binding ratio nγ-CD：^nTY=1：1 and binding constant Kf=495.23 L/mol were confirmed by mole ratio method and linear method. NR was used as molecule probe, and the interaction between γ-CD-TY and herring-sperm DNA was studied by UV spectra, fluorescence spectra, electrochemistry, thermodynamic method and viscosity method, etc. A series of thermodynamic functions such as △rGm^⊙=-2.82×10^4J/mol, △rHm^⊙=5.67×10^4J/mol, △rSm^⊙=284.78J/（mol·K） were obtained, and △rGm^⊙ is the primary driven power of this o interaction, the binding ratio is nγ-CD-TY^nDNA=6：1, the binding constants are K298.15K^⊙=1.12×10^5L/mol, K310.15K^⊙=2.72×10^5L/mol. The interaction mode between γ-CD-TY and herring-sperm DNA is a mixed binding, consisting of partly intercalation and dis-intercalation.