中文摘要：

在pH7．4的tris—HCl溶液中，Gd（Ⅲ）与铬蓝黑R（EBBR）生成1：3的配合物，配合物与鲱鱼精DNA形成5：1的复合物Gd（Ⅲ）（EBBR）3-DNA．通过双倒数法和热力学公式计算得到复合物Gd（Ⅲ）（EBBR）3-DNA在不同温度下的结合常数和其他的热力学常数．结合探针法、碱基模拟、Scatchard法和黏度法表明Gd（Ⅲ）（EBBR）。与DNA是通过嵌插作用和沟槽作用形成复合物Gd（Ⅲ）（EBBR）3-DNA．配合物Gd（Ⅲ）（EBBR）。与鲱鱼精DNA形成复合物的过程是由焓和熵共同驱动．

英文摘要：

The binding ratio between Gd（Ⅲ） and eriochrome blue black R（EBBR） in tris-HCl solution （pH 7.4） was determined as nGd（Ⅲ） ： nEBBR = 1 ： 3, and the binding ratio between Gd（ m ） （EBBR）3 and DNA was determined as 5 ： 1. By double reciprocal method and thermodynamic formulae, the binding constants of Gd（ Ⅲ） （EBBR）3-DNA at dif- ferent temperature and other thermodynamic constants were confirmed. Combined with probe method, basic group simulation, Scatchard method and viscosity method, the results suggest that the interaction mode between Gd（ Ⅲ ）（EBBR）3 complex and herring sperm DNA are intercalation binding and groove binding. Entropy and enthalpy are the primary driving power of the interaction of Gd（ Ⅲ） （EBBR）3 complex and herring sperm DNA.